Characterization of the small RNA transcriptomes of cell protrusions and cell bodies of highly metastatic hepatocellular carcinoma cells via RNA sequencing
| Autor: | Jin Yu, Biting Wu, Tong Qingchao, Ji Jingzhang, Zhifa Shen, Ping Ren, Wenpin Cai, Lihong Yang, Kaixuan Hao |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Cancer Research Small RNA Cell Biology Boyden chamber assay Transcriptome 03 medical and health sciences 0302 clinical medicine Circular RNA microRNA medicine metastasis Gene HCCLM3 cells RNA circular RNA cell body Articles hepatocellular carcinoma cell protrusion Molecular biology Fold change 030104 developmental biology medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis |
| Zdroj: | Oncology Letters |
| ISSN: | 1792-1082 1792-1074 |
| DOI: | 10.3892/ol.2021.12829 |
| Popis: | Increasing evidence suggest that hepatocellular carcinoma (HCC) HCCLM3 cells initially develop pseudopodia when they metastasize, and microRNAs (miRNAs/miRs) and circular RNAs (circRNAs) have been demonstrated to serve important roles in the development, progression and metastasis of cancer. The present study aimed to isolate the cell bodies (CBs) and cell protrusions (CPs) from HCCLM3 cells, and screen the miRNAs and circRNAs associated with HCC infiltration and metastasis in CBs and CPs. The Boyden chamber assay has been confirmed to effectively isolate the CBs and CPs from HCCLM3 cells via observation of microtubule immunofluorescence, DAPI staining and nuclear protein H3 western blotting. Following high-throughput sequencing of the successfully isolated CBs and CPs, 64 pairs of miRNAs, including 23 pairs of upregulated genes and 41 pairs of downregulated genes, and 260 sets of circRNAs, including 127 upregulated genes and 133 downregulated genes, were significantly differentially expressed, using the following criteria: HP/HB ratio, fold change ≥|1.5|, P |
| Databáze: | OpenAIRE |
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