Increased expression of angiogenic cytokines in CD56+ uterine natural killer cells from women with recurrent miscarriage
| Autor: | Jacqueline Pui Wah Chung, Xiaoyan Chen, Yiwei Zhao, Yingyu Liu, Tin-Chiu Li, Jin Huang, Wing Ching Cheung, Chi Chiu Wang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Adult
0301 basic medicine Abortion Habitual Angiogenin Angiogenesis Biopsy medicine.medical_treatment Immunology Cell Count Cytokine Expression Profile Endometrium Biochemistry Andrology 03 medical and health sciences 0302 clinical medicine Pregnancy Recurrent miscarriage medicine Humans Immunology and Allergy Embryo Implantation Molecular Biology 030219 obstetrics & reproductive medicine medicine.diagnostic_test business.industry Uterus Hematology medicine.disease CD56 Antigen Killer Cells Natural 030104 developmental biology medicine.anatomical_structure Cytokine embryonic structures Cytokines Angiogenesis Inducing Agents Female business Endometrial biopsy Hormone |
| Zdroj: | Cytokine. 110:272-276 |
| ISSN: | 1043-4666 |
| DOI: | 10.1016/j.cyto.2018.01.013 |
| Popis: | Objective To compare the expression pattern of angiogenic cytokines in CD56+ uNK cells from peri-implantation endometrium in women with a history of recurrent miscarriage and fertile controls. Methods 28 women were recruited, from which 18 women were diagnosed as recurrent miscarriage and 10 women were of proven fertility. Endometrial biopsy samples were obtained precisely 7 days after luteinization hormone surge in a natural cycle. The angiogenic profile of isolated CD56+ uNK cells was determined by RayBio human angiogenesis antibody array G Series 1000. Differentially expressed angiogenic cytokines between groups were validated by ELISA kits. Results CD56+ uNK cells freshly isolated from peri-implantation endometrium were determined to be >90% pure. Angiogenic cytokine array demonstrated that CD56+ uNK cells are one of the angiogenic factors producers in endometrium around the time of embryo implantation. A differential angiogenic cytokine expression profile was found between two groups, with significantly higher expressions of angiogenin, VEGF-A and bFGF in CD56+ uNK cells from women with recurrent miscarriage, compared with fertile controls. Conclusions Differential angiogenic cytokine profile of isolated CD56+ uNK cells suggested the role of uNK cells in the altered endometrial vascularity at the time of implantation, which may account for the endometrial contribution to recurrent miscarriage. |
| Databáze: | OpenAIRE |
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