Whole body hyperthermia selectively induces heat shock protein 72 in neurons of the rat spinal cord
| Autor: | Tomoko Kawai, Takaaki Ikata, Kyoichi Kishi, Shinsuke Katoh, Hong Xia, Shin-ichiro Saito, Kazuhito Rokutan |
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| Rok vydání: | 1998 |
| Předmět: |
Male
Hyperthermia Fever Central nervous system HSP72 Heat-Shock Proteins Biology Heat shock protein Immersion medicine Animals Humans RNA Messenger Northern blot Rats Wistar Heat shock Spinal cord injury Heat-Shock Proteins Motor Neurons Neurons General Neuroscience Anatomy Blotting Northern medicine.disease Spinal cord Immunohistochemistry Rats Cell biology medicine.anatomical_structure Spinal Cord Neuron |
| Zdroj: | Neuroscience Letters. 258:151-154 |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/s0304-3940(98)00876-3 |
| Popis: | The aims of this study were to examine whether the heat shock response is operative in the spinal cord, and to identify the type of responsible cell. Immunoblot analysis using an antibody specific for a highly stress-inducible heat shock protein with a molecular mass of 72 kDa (HSP72) showed that exposing rats to whole body hyperthermia remarkably induced HSP72 protein in the spinal cord within 2 h. Northern blot analysis with a cDNA probe for human HSP72 demonstrated that whole body hyperthermia induced the expression of HSP72 mRNA within 30 min in the spinal cord. Immunohistochemical analysis showed that neurons in the gray matter appear to be a preferential target of the heat shock response, suggesting that the heat shock response might have a therapeutic implication for protection against spinal cord injury. |
| Databáze: | OpenAIRE |
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