Distinct interacting core taxa in co-occurrence networks enable discrimination of polymicrobial oral diseases with similar symptoms
| Autor: | Noriko Maruyama, Kazunori Murase, Fumito Maruyama, Takayasu Watanabe, Tatsuro Koyanagi, Yuichi Izumi, Yasuo Takeuchi, Hirokazu Kachi, Takahiko Shiba, Ichiro Nakagawa |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Pathology medicine.medical_specialty Microbial Consortia Virulence Functional genes Biology DNA Ribosomal Virulence factor Article 03 medical and health sciences 0302 clinical medicine Abundance (ecology) RNA Ribosomal 16S medicine Cluster Analysis Humans In Situ Hybridization Periodontal Diseases Phylogeny Aged Periodontitis Genetics Multidisciplinary Bacteria Coinfection 030206 dentistry Sequence Analysis DNA Middle Aged medicine.disease 16S ribosomal RNA 030104 developmental biology Taxon Female Metagenomics Co-occurrence networks |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Polymicrobial diseases, which can be life threatening, are caused by the presence and interactions of multiple microbes. Peri-implantitis and periodontitis are representative polymicrobial diseases that show similar clinical symptoms. To establish a means of differentiating between them, we compared microbial species and functional genes in situ by performing metatranscriptomic analyses of peri-implantitis and periodontitis samples obtained from the same subjects (n = 12 each). Although the two diseases differed in terms of 16S rRNA-based taxonomic profiles, they showed similarities with respect to functional genes and taxonomic and virulence factor mRNA profiles. The latter—defined as microbial virulence types—differed from those of healthy periodontal sites. We also showed that networks based on co-occurrence relationships of taxonomic mRNA abundance (co-occurrence networks) were dissimilar between the two diseases. Remarkably, these networks consisted mainly of taxa with a high relative mRNA-to-rRNA ratio, with some showing significant co-occurrence defined as interacting core taxa, highlighting differences between the two groups. Thus, peri-implantitis and periodontitis have shared as well as distinct microbiological characteristics. Our findings provide insight into microbial interactions in polymicrobial diseases with unknown etiologies. |
| Databáze: | OpenAIRE |
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