Fibroblast growth factor 10 is a negative regulator of postnatal neurogenesis in the mouse hypothalamus
Autor: | Marta Mikolajczak, Ritva Rice, Mohammad K. Hajihosseini, Shaun J. Clare, Suzanne L. Mansour, Stuart G. Nayar, Saverio Bellusci, Timothy Goodman |
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Přispěvatelé: | HUS Head and Neck Center, Institute of Biotechnology |
Rok vydání: | 2020 |
Předmět: |
Male
GENES Postnatal neurogenesis Mouse Neurogenesis Ependymoglial Cells Population Cell Hypothalamus Mice Transgenic NEURAL STEM-CELLS Biology Lineage tracing Fibroblast growth factor ADULT NEUROGENESIS Mice 03 medical and health sciences Cell movement 0302 clinical medicine Neural Stem Cells medicine Animals BRAIN Progenitor cell education NEURONS Molecular Biology 030304 developmental biology 0303 health sciences education.field_of_study FGF10 Tanycytes 1184 Genetics developmental biology physiology Stem Cells and Regeneration Neural stem cell Cell biology Fibroblast growth factors medicine.anatomical_structure POPULATIONS Female Fibroblast Growth Factor 10 Neuroglia 030217 neurology & neurosurgery Ex vivo Developmental Biology |
Zdroj: | Development (Cambridge, England) article-version (VoR) Version of Record |
ISSN: | 1477-9129 0950-1991 |
Popis: | New neurons are generated in the postnatal rodent hypothalamus, with a subset of tanycytes in the third ventricular (3V) wall serving as neural stem/progenitor cells. However, the precise stem cell niche organization, the intermediate steps and the endogenous regulators of postnatal hypothalamic neurogenesis remain elusive. Quantitative lineage-tracing in vivo revealed that conditional deletion of fibroblast growth factor 10 (Fgf10) from Fgf10-expressing β-tanycytes at postnatal days (P)4-5 results in the generation of significantly more parenchymal cells by P28, composed mostly of ventromedial and dorsomedial neurons and some glial cells, which persist into adulthood. A closer scrutiny in vivo and ex vivo revealed that the 3V wall is not static and is amenable to cell movements. Furthermore, normally β-tanycytes give rise to parenchymal cells via an intermediate population of α-tanycytes with transient amplifying cell characteristics. Loss of Fgf10 temporarily attenuates the amplification of β-tanycytes but also appears to delay the exit of their α-tanycyte descendants from the germinal 3V wall. Our findings suggest that transience of cells through the α-tanycyte domain is a key feature, and Fgf10 is a negative regulator of postnatal hypothalamic neurogenesis. Summary: Generation of new hypothalamic neurons after birth is a multistep process involving cell division and cell movements that are controlled by Fgf10. |
Databáze: | OpenAIRE |
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