Mesenchymal Stem Cells Provide Better Results Than Hematopoietic Precursors for the Treatment of Myocardial Infarction
| Autor: | J. Manuel Garcia-Verdugo, J. Anastasio Montero, Pilar Solves, Rafael Payá, Carolina Gandía, María Dolores Miñana, Pilar Sepúlveda, Elisa Lledó, Amparo Ruiz-Sauri, César Trigueros, Ana Armiñán |
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| Rok vydání: | 2010 |
| Předmět: |
mesenchymal stem cells
Pathology medicine.medical_specialty business.industry medicine.medical_treatment Mesenchymal stem cell CD34 hematopoietic precursors Hematopoietic stem cell transplantation medicine.disease Transplantation left ventricular function Haematopoiesis myocardial infarction medicine.anatomical_structure Immunology medicine Myocardial infarction paracrine factors Stem cell Cardiology and Cardiovascular Medicine business Artery |
| Zdroj: | JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY r-CIPF: Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF) Centro de Investigación Principe Felipe (CIPF) r-CIPF. Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF) instname |
| ISSN: | 0735-1097 |
| Popis: | Objectives The purpose of this study was to compare the ability of human CD34(+) hematopoietic stem cells and bone marrow mesenchymal stem cells (MSC) to treat myocardial infarction (MI) in a model of permanent left descendent coronary artery (LDA) ligation in nude rats. Background Transplantation of human CD34(+) cells and MSC has been proved to be effective in treating MI, but no comparative studies have been performed to elucidate which treatment prevents left ventricular (LV) remodelling more efficiently. Methods Human bone marrow MSC or freshly isolated CD34(+) cells from umbilical cord blood were injected intramyocardially in infarcted nude rats. Cardiac function was analyzed by echocardiography. Ventricular remodelling was evaluated by tissue histology and electron microscopy, and neo-formed vessels were quantified by immunohistochemistry. Chronic local inflammatory infiltrates were evaluated in LV wall by hematoxylin-eosin staining. Apoptosis of infarcted tissue was evaluated by terminal deoxynucleotidyl transferase dUTP nick end labeling assay. Results Both cell types induced an improvement in LV cardiac function and increased tissue cell proliferation in myocardial tissue and neoangiogenesis. However, MSC were more effective for the reduction of infarct size and prevention of ventricular remodelling. Scar tissue was 17.48 +/- 1.29% in the CD34 group and 10.36 +/- 1.07% in the MSC group (p < 0.001 in MSC vs. CD34). Moreover, unlike MSC, CD34(+)-treated animals showed local inflammatory infiltrates in LV wall that persisted 4 weeks after transplantation. Conclusions Mesenchymal stem cells might be more effective than CD34(+) cells for the healing of the infarct. This study contributes to elucidate the mechanisms by which these cell types operate in the course of MI treatment. (J Am Coll Cardiol 2010;55:2244-53) (C) 2010 by the American College of Cardiology Foundation |
| Databáze: | OpenAIRE |
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