Centimeter-Scale Surface Interactions Using Hydrodynamic Flow Confinements
| Autor: | Ismael Zeaf, David P. Taylor, Robert D. Lovchik, Govind V. Kaigala |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Surface (mathematics) Scale (ratio) Chemistry Numerical analysis Microfluidics Nanotechnology 02 engineering and technology Surfaces and Interfaces Chemical interaction Substrate (electronics) 021001 nanoscience & nanotechnology Condensed Matter Physics Article 03 medical and health sciences 030104 developmental biology Electrochemistry General Materials Science 0210 nano-technology Biological system Spectroscopy Hydrodynamic flow |
| Zdroj: | Langmuir |
| ISSN: | 1520-5827 0743-7463 |
| Popis: | We present a device and method for selective chemical interactions with immersed substrates at the centimeter-scale. Our implementations enable both, sequential and simultaneous delivery of multiple reagents to a substrate, as well as the creation of gradients of reagents on surfaces. The method is based on localizing submicroliter volumes of liquids on an immersed surface with a microfluidic probe (MFP) using a principle termed hydrodynamic flow confinement (HFC). We here show spatially defined, multiplexed surface interactions while benefiting from the probe capabilities such as non-contact scanning operation and convection-enhanced reaction kinetics. Three-layer glass-Si-glass probes were developed to implement slit-aperture and aperture-array designs. Analytical and numerical analysis helped to establish probe designs and operating parameters. Using these probes, we performed immunohistochemical analysis on individual cores of a human breast-cancer tissue microarray. We applied α-p53 antibodies on a 2 mm diameter core within 2.5 min using a slit-aperture probe (HFC dimension: 0.3 mm × 1.2 mm). Further, multiplexed treatment of a tissue core with α-p53 and α-β-actin antibodies was performed using four adjacent HFCs created with an aperture-array probe (HFC dimension: 4 × 0.3 mm × 0.25 mm). The ability of these devices and methods to perform multiplexed assays, present sequentially different liquids on surfaces, and interact with surfaces at the centimeter-scale will likely spur new and efficient surface assays. |
| Databáze: | OpenAIRE |
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