Development of American-Lineage Influenza H5N2 Reassortant Vaccine Viruses for Pandemic Preparedness

Autor:	Min-Shi Lee, Po-Ling Chen, Min-Yuan Chia, Chia-Tsui Yeh, Alan Yung-Chih Hu, Chun-Yang Lin, Wen-Chin Lin, Ming-Chu Cheng, Tsai-Chuan Weng, Chia-Chun Lai, Yu-Fen Tseng, Ih-Jen Su
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	0301 basic medicine H5N1 vaccine viruses American-lineage reassortant influenza viruses Taiwan lcsh:QR1-502 Neuraminidase Hemagglutinin Glycoproteins Influenza Virus Biology medicine.disease_cause Antibodies Viral Virus Article lcsh:Microbiology 03 medical and health sciences Viral Proteins 0302 clinical medicine Virology Reassortant Viruses Pandemic Chlorocebus aethiops Influenza Human medicine Animals Humans 030212 general & internal medicine American-lineage H5N2 vaccine Vero Cells Ferrets Influenza A virus subtype H5N1 Reverse genetics Reverse Genetics 030104 developmental biology Infectious Diseases Treatment Outcome Immunization Vaccines Inactivated Influenza Vaccines Vero cell Influenza A Virus H5N2 Subtype Pandemic preparedness
Zdroj:	Viruses, Vol 11, Iss 6, p 543 (2019) Viruses Volume 11 Issue 6
ISSN:	1999-4915
Popis:	Novel low-pathogenic avian influenza (LPAI) H5N2 viruses hit poultry farms in Taiwan in 2003, and evolved into highly pathogenic avian influenza (HPAI) viruses in 2010. These viruses are reassortant viruses containing HA and NA genes from American-lineage H5N2 and six internal genes from local H6N1 viruses. According to a serological survey, the Taiwan H5N2 viruses can cause asymptomatic infections in poultry workers. Therefore, a development of influenza H5N2 vaccines is desirable for pandemic preparation. In this study, we employed reverse genetics to generate a vaccine virus having HA and NA genes from A/Chicken/CY/A2628/2012 (E7, LPAI) and six internal genes from a Vero cell-adapted high-growth H5N1 vaccine virus (Vero-15). The reassortant H5N2 vaccine virus, E7-V15, presented high-growth efficiency in Vero cells (512 HAU, 107.6 TCID50/mL), and passed all tests for qualification of candidate vaccine viruses. In ferret immunization, two doses of inactivated whole virus antigens (3 &mu g of HA protein) adjuvanted with alum could induce robust antibody response (HI titre 113.14). In conclusion, we have established reverse genetics to generate a qualified reassortant H5N2 vaccine virus for further development.
Databáze:	OpenAIRE
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