Combination of 16S rRNA and procalcitonin in diagnosis of neonatal clinically suspected sepsis
| Autor: | Renqiang Yu, Min Wang, Qin Zhou, Shanyu Jiang, Yingzi Mei |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Medicine (General) medicine.medical_specialty neonatal sepsis diagnosis 030106 microbiology blood culture Biochemistry Gastroenterology Sensitivity and Specificity Procalcitonin Sepsis 03 medical and health sciences R5-920 Internal medicine RNA Ribosomal 16S medicine Escherichia coli Humans Blood culture 16S rRNA Neonatal sepsis medicine.diagnostic_test business.industry Biochemistry (medical) Infant Newborn Clinical Research Report Cell Biology General Medicine medicine.disease 16S ribosomal RNA 030104 developmental biology business |
| Zdroj: | The Journal of International Medical Research Journal of International Medical Research, Vol 48 (2020) |
| ISSN: | 1473-2300 0300-0605 |
| Popis: | Objective To investigate the application of 16S rRNA in diagnosing patients with neonatal sepsis. Methods We studied 60 consecutive neonatal patients with clinically suspected sepsis and 20 non-infective cases as controls. All patients were diagnosed with sepsis by clinical and experimental criteria. Clinical characteristics were recorded and 16S rRNA sequencing was conducted for all patients. The sensitivity, specificity, and accuracy of the detection methods were analyzed. Results The detection limit of 16S rRNA sequencing was 1 × 102 CFU/mL. For suspected sepsis, the positive rate of 16S rRNA detection was 93.3%, which was similar to that of procalcitonin detection (85%), and was significantly higher than that of bacterial culture (51.7%). The specificity of procalcitonin detection (74.1%) was significantly lower than that of 16S rRNA detection (100%). Moreover, the combination of 16S rRNA and procalcitonin detection showed a sensitivity of 100%, specificity of 74.1%, and accuracy of 92.0%. For proven sepsis, the sensitivity and specificity of 16S rRNA detection were both 100.0%, and those for procalcitonin were 87.1% and 87.0%, respectively. Conclusion Detection of 16S rRNA has high sensitivity and specificity in diagnosing sepsis. The combination of 16S rRNA and procalcitonin has even better sensitivity with acceptable specificity. |
| Databáze: | OpenAIRE |
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