Performance of a sandwich antigen-detection ELISA for the diagnosis of porcine Taenia solium cysticercosis
| Autor: | Susan Y Yang, Armando E. Gonzalez, Hector H. Garcia, Pierre Dorny, Sarah Gabriël, Berenice Ninaquispe, Robert H. Gilman, Silvia Rodriguez, Yesenia Castillo, Javier A. Bustos |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
pig
Taenia hydatigena Swine Neurocysticercosis NEUROCYSTICERCOSIS SERUM parasite load 0302 clinical medicine Taenia solium PIGS cross reaction Swine Diseases biology Intermediate host Cysticercosis Articles unclassified drug PREVALENCE medicine.drug_formulation_ingredient Infectious Diseases INFECTIONS disease severity purl.org/pe-repo/ocde/ford#3.03.06 [https] taeniasis EXCRETORY-SECRETORY ANTIGENS animal experiment 030231 tropical medicine UNITED-STATES Enzyme-Linked Immunosorbent Assay Sensitivity and Specificity Article 03 medical and health sciences Antigen Virology parasitic diseases monoclonal antibody 60H8 medicine follow up Animals Helminths controlled study Veterinary Sciences ANTIBODY-BASED ELISA Taenia hydatigena infection nonhuman business.industry animal model cysticercosis treatment response biology.organism_classification medicine.disease enzyme linked immunosorbent assay monoclonal antibody 158C11 GLYCOPROTEINS echinococcosis experimental infection Parasitology monoclonal antibody sensitivity and specificity Antigens Helminth CIRCULATING ANTIGEN business |
| Zdroj: | AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE |
| ISSN: | 0002-9637 1476-1645 |
| Popis: | The pig is the natural intermediate host of Taenia solium, a parasite causing significant burden of disease in both humans and pigs. Porcine cysticercosis is traditionally detected via tongue palpation and slaughterhouse meat inspection, both with limited sensitivity. Serum antibody detection has a better performance; however, it does not discriminate past from present infection. Serum antigen detection can demonstrate viable infection and gives a good estimate of parasitic load. This study evaluated a sandwich antigen-detection ELISA using monoclonal antibodies (MoAbs) 158C11 and 60H8 for the diagnosis of viable cysticercosis in pigs. Serum samples were used from 35 naturally T. solium cysticerci–infected pigs, 31 cysticercosis-negative pigs, and 22 pigs with Taenia hydatigena infection (to assess cross-reactions). Positive cysticercosis samples were subcategorized at necropsy according to parasitic burden as mild (1–10 viable cysts, n = 10), moderate (11–100 cysts, n = 5), or severe infection (more than 100 cysts, n = 20). This Ag-ELISA showed a sensitivity of 82.9% and a specificity of 96.8% when not considering cross-reactions with T. hydatigena. Hundred percentage of severely infected, 80% of moderately infected, and 50% of mildly T. solium–infected pigs tested positive. Twenty of 22 pigs with only T. hydatigena infections were positive, with 13 reaching saturating levels in the ELISA. The Ag-ELISA revealed the presence of live cysts and is, thus, a fairly reliable test to monitor experimental infection, response to treatment, and follow-up in animal models of cysticercosis. It should, however, be carefully interpreted when used in regions where T. hydatigena is endemic in pigs. |
| Databáze: | OpenAIRE |
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