Ultraviolet-B radiation effects on phenolic profile and flavonoid content of Kalanchoe pinnata
| Autor: | Virgínia Rodrigues Lopes, Eliana Schwartz Tavares, Sônia Soares Costa, Marcos Vinicius Leal-Costa, Luana Beatriz dos Santos Nascimento, Eloá Aragão Menezes, Michelle Frazão Muzitano |
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| Rok vydání: | 2015 |
| Předmět: |
Kalanchoe
Antioxidant Ultraviolet Rays medicine.medical_treatment Flavonoid Biophysics High-performance liquid chromatography Antioxidants chemistry.chemical_compound Phenols Botany medicine Radiology Nuclear Medicine and imaging Food science Chromatography High Pressure Liquid Flavonoids chemistry.chemical_classification Radiation Radiological and Ultrasound Technology biology Plant Extracts fungi food and beverages biology.organism_classification Quercitrin Crassulaceae Plant Leaves chemistry Polyphenol Composition (visual arts) Chromatography Thin Layer |
| Zdroj: | Journal of Photochemistry and Photobiology B: Biology. 148:73-81 |
| ISSN: | 1011-1344 |
| Popis: | Ultraviolet-B radiation is an important abiotic factor that can stimulate the production of secondary metabolites, including polyphenolic compounds. Kalanchoe pinnata (Crassulaceae) is a medicinal plant popularly used in Brazil for treating wounds and inflammation. This species is rich in phenolic compounds, which could account for some of its biological activities, including antileishmanial, antihypertensive and antibacterial properties. We investigated the effects of supplemental UV-B radiation on the phenolic profile, antioxidant activity and total flavonoid content of leaves of K. pinnata. Plants were grown under white light (W - control) and supplemental UV-B radiation (W+UVB). Supplemental UV-B radiation enhanced the total flavonoid content of the leaf extracts, without affecting the antioxidant activity or yield of extracts. Analysis by TLC and HPLC of W and W+UVB leaf extracts revealed quantitative and qualitative differences in their phenolic profiles. W+UVB extracts contained a higher diversity of phenolic compounds and a larger amount of quercitrin, an important bioactive flavonoid of this species. This is the first report of the use of ImageJ® program to analyze a TLC visualized by spraying with NP-PEG reagent. UV-B radiation is proposed as a supplemental light source in K. pinnata cultivation in order to improve its flavonoid composition. |
| Databáze: | OpenAIRE |
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