Expression of functional molecules by human CD3- decidual granular leucocyte clones
| Autor: | Peter M. Johnson, Daniel Rukavina, Günnur Deniz, Lea Gudelj, Stephen E. Christmas |
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| Rok vydání: | 1996 |
| Předmět: |
Cytoplasm
CD3 Complex medicine.drug_class CD3 Immunology Cell Culture Techniques Complement receptor CD16 Monoclonal antibody Immunophenotyping Antigen Pregnancy medicine Decidua Leukocytes Immunology and Allergy Cytotoxic T cell Humans Receptor biology Receptors IgG Flow Cytometry Molecular biology Clone Cells Receptors Complement Killer Cells Natural Antigens Surface biology.protein Female Research Article |
| Zdroj: | Immunology. 87(4) |
| ISSN: | 0019-2805 |
| Popis: | Cell surface and cytoplasmic antigen expression by 35 CD3- decidual granular leucocyte (DGL) clones, derived from human endometrial tissue in the first trimester of pregnancy, has been compared with both that of fresh CD3- decidual leucocytes and that of CD3- peripheral blood natural killer (PBNK) cell clones (n = 12). The majority of DGL clones retained the antigenic phenotype of fresh cells, although CD103 (HML-1) was expressed on 50% of DGL clones but only 17% of fresh DGL. Both cytoplasmic CD3 zeta and CD3 epsilon chains were detected in > 90% of DGL clones in the absence of cell surface CD3. Cytoplasmic CD3 zeta was present in almost all fresh CD3- DGL, whereas CD3 epsilon was not. Most DGL clones did not express surface Fc gamma receptors I-III (CD64, -32 and -16, respectively) and complement receptors (CR) types 1 and 2 (CD35 and 21, respectively), but 43% expressed CR3 (CD11b/18); in contrast, all PBNK clones were CR3+. The NK cell-associated molecules Kp43 (CD94) and the p58 molecule recognized by the HP3E4 monoclonal antibody were both present on a higher proportion of CD3- PBNK (91% and 50%, respectively) than DGL clones (31% and 14%, respectively), despite expression of CD94 by > 90% of fresh CD56+ decidual leucocytes. Five of 35 CD3- DGL clones expressed cytoplasmic CD3 zeta in the absence of expression of CD2, CD16 or the p58 molecule recognized by HP3E4. These variations between CD3- DGL and PBNK cell clones in expression of functional molecules may be related to previously reported differences in major histocompatibility complex-non-restricted cytotoxic activities between these two cell types. |
| Databáze: | OpenAIRE |
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