RecombinantBacillus subtilisspores expressing MPT64 evaluated as a vaccine against tuberculosis in the murine model
| Autor: | Simon M. Cutting, David S. Radford, Rajko Reljic, Jen-Min Huang, Laura Sibley, Huynh A. Hong, Rocky M. Cranenburgh |
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| Rok vydání: | 2014 |
| Předmět: |
Enzyme-Linked Immunospot Assay
Recombinant Fusion Proteins T-Lymphocytes Genetic Vectors Chimeric gene Bacillus subtilis Microbiology law.invention Mice Bacterial Proteins Antigen law Genetics Animals Tuberculosis Tuberculosis Vaccines Lung Molecular Biology Spores Bacterial Antigens Bacterial Drug Carriers Vaccines Synthetic Attenuated vaccine biology ELISPOT Vaccination fungi biology.organism_classification Virology Bacterial Load Spore Mice Inbred C57BL Disease Models Animal Vaccines Inactivated Recombinant DNA Cytokines Female Cell Surface Display Techniques Tuberculosis vaccines |
| Zdroj: | FEMS Microbiology Letters. 358:170-179 |
| ISSN: | 0378-1097 |
| DOI: | 10.1111/1574-6968.12525 |
| Popis: | Recombinant Bacillus subtilis spores expressing a TB antigen, MPT64, were tested for their ability to protect mice against tuberculosis challenge. A chimeric gene consisting of the spore coat gene cotB fused to mpt64 was constructed, and expression of a stable CotB-MPT64 hybrid protein of the spore coat verified. Spores were evaluated as a live vaccine and also formaldehyde inactivated. Mice were given three doses of spores or alternatively used in a prime-boost regimen with BCG. The results showed that inactivated recombinant spores were able to reduce the bacterial burden in the lungs of mice to comparable levels to that of BCG. In the prime-boost regimen, both live and inactivated spores showed a reduction in bacterial load in comparison with BCG. ELISPOT and polyfunctional T-cell analysis were performed to examine cellular responses and showed that antigen-specific secretion of Th1 cytokines was stimulated after immunisation with inactive recombinant spores and BCG. In summary, recombinant spores can elicit Th1 responses, which are important for protection against TB disease. |
| Databáze: | OpenAIRE |
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