Flow cytometry and cytomorphology evaluation of hematologic malignancy in cerebrospinal fluids: comparison with retrospective clinical outcome
| Autor: | Giambattista Bertani, Catherine Klersy, Annamaria Nosari, Barbara Scarpati, Claudia Barba, Giovanni Grillo, Clara Cesana, Linda Scampini, Ursula Ferri, Bruno Brando, Enrica Morra, Giuliana Lando, Arianna Gatti, Elisabetta Volpato, Maurizio Faleri, Roberto Cairoli |
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| Přispěvatelé: | Cesana, C, Klersy, C, Scarpati, B, Brando, B, Faleri, M, Bertani, G, Gatti, A, Volpato, E, Barba, C, Ferri, U, Scampini, L, Grillo, G, Lando, G, Nosari, A, Morra, E, Cairoli, R |
| Rok vydání: | 2010 |
| Předmět: |
Male
medicine.medical_specialty Pathology Hematologic malignancy Lymphoid neoplasm Cytodiagnosis Intrathecal Gastroenterology Sensitivity and Specificity Flow cytometry Immunophenotyping Internal medicine Medicine Humans Lymphoid neoplasms Cytomorphology Combined method Cerebrospinal Fluid Retrospective Studies Observation time Hematology medicine.diagnostic_test business.industry General Medicine Flow Cytometry Treatment Outcome Hematologic Neoplasms Female business Clinical evaluation |
| Zdroj: | Annals of hematology. 90(7) |
| ISSN: | 1432-0584 |
| Popis: | An independent clinical assessment was compared with flow cytometry (FCM) and cytomorphology results obtained on 227 cerebrospinal fluids investigated for hematologic malignancy, in a retrospective longitudinal study with a median observation time of 11 months. A combined method assessment (CMA), defining "positive" a sample if at least one method gave "positive" results, was also tested. Eleven out of 55 screening samples and 53 out of 166 follow-up samples resulted positive at clinical evaluation. FCM and CM were concordant with positive clinical assessment in 68.5% and 51.5% of cases, respectively. According to CMA, 10.5% of samples (resulting false negative by either FCM or cytomorphology) were rescued as true positive. FCM retained significantly higher accuracy than cytomorphology (p = 0.0065) and 100% sensitivity when at least 220 leukocytes were acquired. CMA accuracy was higher than FCM accuracy and significantly higher than cytomorphology accuracy in the analysis of all samples (p < 0.0001), samples from mature B/T cell neoplasms (p = 0.0021), and samples drawn after intrathecal treatment (p = 0.0001). When acquiring ≥220 leukocytes, FCM accuracy was poor, and combining cytomorphology added statistically significant diagnostic advantage (p = 0.0043). Although FCM is the best diagnostic tool for evaluating CSF, morphology seems helpful especially when clinically positive follow-up samples are nearly acellular. |
| Databáze: | OpenAIRE |
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