Creatine kinase B is necessary to limit myoblast fusion during myogenesis
| Autor: | Yanru Wang, Christophe Pichavant, James M. Ervasti, Adriana Simionescu-Bankston, Grace K. Pavlath, Luciano H. Apponi, John T. Olthoff, James P. Canner, Joseph J. Belanto, Craig M Steeds |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Physiology
Muscle Fibers Skeletal Primary Cell Culture Biology Muscle Development Polymerization Cell Fusion Myoblasts Mice Myoblast fusion Two-Hybrid System Techniques Creatine Kinase BB Form Animals Protein Isoforms Myocyte RNA Small Interfering Actin Mice Inbred BALB C Gene knockdown Cell fusion Myogenesis Creatine Kinase MM Form Gene Expression Regulation Developmental Articles Cell Biology Actins Transport protein Cell biology Mice Inbred C57BL Alternative Splicing Protein Transport Signal transduction Signal Transduction |
| Zdroj: | American Journal of Physiology-Cell Physiology. 308:C919-C931 |
| ISSN: | 1522-1563 0363-6143 |
| DOI: | 10.1152/ajpcell.00029.2015 |
| Popis: | Myoblast fusion is critical for proper muscle growth and regeneration. During myoblast fusion, the localization of some molecules is spatially restricted; however, the exact reason for such localization is unknown. Creatine kinase B (CKB), which replenishes local ATP pools, localizes near the ends of cultured primary mouse myotubes. To gain insights into the function of CKB, we performed a yeast two-hybrid screen to identify CKB-interacting proteins. We identified molecules with a broad diversity of roles, including actin polymerization, intracellular protein trafficking, and alternative splicing, as well as sarcomeric components. In-depth studies of α-skeletal actin and α-cardiac actin, two predominant muscle actin isoforms, demonstrated their biochemical interaction and partial colocalization with CKB near the ends of myotubes in vitro. In contrast to other cell types, specific knockdown of CKB did not grossly affect actin polymerization in myotubes, suggesting other muscle-specific roles for CKB. Interestingly, knockdown of CKB resulted in significantly increased myoblast fusion and myotube size in vitro, whereas knockdown of creatine kinase M had no effect on these myogenic parameters. Our results suggest that localized CKB plays a key role in myotube formation by limiting myoblast fusion during myogenesis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|