Coordinate changes of renin and brain-type nitric-oxide-synthase (b-NOS) mRNA levels in rat kidneys
| Autor: | Bernhard Pötzl, Karin Schricker, Armin Kurtz, Marlies Hamann |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Male
medicine.medical_specialty Physiology Clinical Biochemistry Molecular Sequence Data Endothelial NOS Kidney Polymerase Chain Reaction Rats Sprague-Dawley Angiotensin Receptor Antagonists Renal Artery Physiology (medical) Internal medicine medicine.artery Renin–angiotensin system Renin medicine Animals RNA Messenger Salt intake Renal artery biology Base Sequence Angiotensin II Brain Rats Nitric oxide synthase Endocrinology medicine.anatomical_structure Losartan Hypertension Renovascular biology.protein Nitric Oxide Synthase Atrial Natriuretic Factor medicine.drug |
| Zdroj: | Pflugers Archiv : European journal of physiology. 432(3) |
| ISSN: | 0031-6768 |
| Popis: | In our study we have examined the mRNA levels of nitric-oxide-(NO-)synthases in rat kidneys during states of stimulated and reduced renin gene expression, to find out whether renal mRNA levels of NO-synthases are correlated with the activity of the renin system. Stimulation of the renin system was achieved by unilateral renal artery clipping (2-kidney/1-clip rats), treatment with the angiotensin II (ANG II) antagonist losartan (40 mg/kg), application of furosemide (12 mg x kg-1 x day-1) and a low-sodium diet (0.02% w/w Na+), which increased renin mRNA levels to 464%, 495%, 309% and 219% of those of control animals, respectively. Inhibition of the renin system was achieved in the nonclipped (contralateral) kidneys of 2-kidney/1-clip rats and in the kidneys of rats which were fed a high-sodium diet (4% w/w Na+); in both cases renin mRNA levels decreased to about 50% of the control values. First screening of the gene expression of brain-type NO-synthase (b-NOS), endothelial NOS (e-NOS) and inducible NOS (i-NOS) during all these alterations of the renin system was done using the reverse transcriptase-polymerase chain reaction (RT-PCR) technique. Results from such noncompetitive PCR experiments indicated that only b-NOS mRNA levels change concordantly with the levels of renin. These changes in b-NOS mRNA levels were checked by the more reliable method of RNase protection assay. Results of the RNase protection assay proved that the renal levels of b-NOS mRNA were significantly increased by about 50% after a low-sodium diet and hypoperfusion of the kidney. Given a stimulatory role of endothelium-derived relaxing factor (EDRF)/NO on the renin system our findings may provide the first evidence that increases of renal levels of b-NOS mRNA and, as a consequence, of renal EDRF/NO formation could be important mediators of the well-known effect of salt intake and hypoperfusion on the renin system. |
| Databáze: | OpenAIRE |
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