Molecular Diagnosis of Actinomadura madurae Infection by 16S rRNA Deep Sequencing

Autor:	Lindley A. Barbee, Bronwyn H. Bryant, Stephanie Thielges, Noah G. Hoffman, Catherine Natividad, Dhruba J. Sengupta, Christopher Rosenthal, Brad T. Cookson, Daniel R. Hoogestraat, Stephen J. Salipante, Lisa A. Cummings, Mimosa Chau, Peter W. Monsaas
Rok vydání:	2013
Předmět:	DNA Bacterial Microbiology (medical) Molecular Sequence Data Case Reports DNA Ribosomal Deep sequencing DNA sequencing Bacterial genetics Diabetes Complications Phylogenetics RNA Ribosomal 16S Actinomycetales Cluster Analysis Humans Actinomadura madurae Phylogeny Genetics Microscopy biology Foot Histocytochemistry High-Throughput Nucleotide Sequencing Middle Aged Ribosomal RNA biology.organism_classification 16S ribosomal RNA Mycetoma Female
Zdroj:	Journal of Clinical Microbiology. 51:4262-4265
ISSN:	1098-660X 0095-1137
DOI:	10.1128/jcm.02227-13
Popis:	Next-generation DNA sequencing can be used to catalog individual organisms within complex, polymicrobial specimens. Here, we utilized deep sequencing of 16S rRNA to implicate Actinomadura madurae as the cause of mycetoma in a diabetic patient when culture and conventional molecular methods were overwhelmed by overgrowth of other organisms.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4369956b89dce71166861fa794bb9827 https://doi.org/10.1128/jcm.02227-13 Zobrazit plný text záznamu