Comparison of Antibodies to Detect Uroplakin in Urothelial Carcinomas
| Autor: | Heidi L. Kristoffersen, Rasmus Røge, Søren Nielsen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Male
Carcinoma Transitional Cell Carcinoma Squamous Cell/diagnosis Histology Urothelial carcinomas Antibody clone Antibodies Monoclonal Carcinoma Transitional Cell/diagnosis Immunohistochemistry Pathology and Forensic Medicine Uroplakin Medical Laboratory Technology Mice Urothelium/pathology Urinary Bladder Neoplasms Urinary Bladder Neoplasms/diagnosis Uroplakin II Carcinoma Squamous Cell Animals Humans Female Urothelium External quality control |
| Zdroj: | Kristoffersen, H L, Røge, R & Nielsen, S 2022, ' Comparison of Antibodies to Detect Uroplakin in Urothelial Carcinomas ', Applied Immunohistochemistry & Molecular Morphology, vol. 30, no. 5, pp. 326-332 . https://doi.org/10.1097/PAI.0000000000001021 |
| DOI: | 10.1097/PAI.0000000000001021 |
| Popis: | Immunohistochemistry for Uroplakin (UP) II and III is used to determine urothelial origin of carcinomas of unknown primary site and are especially valuable to differentiate urothelial carcinomas (UCs) from lung squamous cell carcinomas and prostate carcinomas. In the Nordic immunohistochemical Quality Control assessment scheme, only 45% of the participants obtained a sufficient staining result for UP. Primary antibodies (Abs) against UPII were most successful with a pass rate of 86%. No Abs against UPIII provided sufficient staining results. A comparative study was carried out on a larger cohort of tissue samples with optimized methods for the UPII mouse monoclonal antibody (mmAb) clone BC21, UPIII mmAb clone AU-1, and rabbit monoclonal antibody (rmAb) clone SP73 to evaluate the performance in a standardized way. Tissue microarrays containing 58 UCs, 111 non-UCs, and 20 normal tissues were included. The UP stains were evaluated by using H-score. Based on H-scores, samples were categorized as high-expressor (150 to 300), moderate-expressor (10 to 149), low-expressor (1 to 9), and negative (150 for the UPII Ab. The 2 UPIII Abs gave an analytical specificity of 100% compared with 97% for the UPII Ab being positive in 2 ovarian carcinomas and 1 cervical squamous cell carcinoma. |
| Databáze: | OpenAIRE |
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