A glycosyl transferase family 43 protein involved in xylan biosynthesis is associated with straw digestibility in Brachypodium distachyon

Autor: Matthieu Reymond, Assaf Distelfeld, Simon J. McQueen-Mason, Fernando Pistón, Caragh Whitehead, Leonardo D. Gomez, Rachael Simister, Sergio G. Atienza, Francisco Garrido
Přispěvatelé: International Anthony Burgess Foundation, European Commission, Biotechnology and Biological Sciences Research Council (UK), Centre for Novel Agricultural Products, Department of Biology, University of York [York, UK], Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Department of Molecular Biology and Ecology of Plants, Tel Aviv University (TAU), Burgess Foundation, BBSRC [BB/G016178, BB/G016194], European Project: 211982,EC:FP7:KBBE,FP7-KBBE-2007-1,RENEWALL(2008), Whitehead, Caragh, Ostos Garrido, Francisco J., Gomez, Leonardo D., McQueen-Mason, Simon J., Tel Aviv University [Tel Aviv]
Rok vydání: 2017
Předmět:
0106 biological sciences
0301 basic medicine
Candidate gene
Physiology
GT43
Plant Science
01 natural sciences
xylan
Animals
Genetically Modified
gene silencing
Cell Wall
Gene Expression Regulation
Plant
Inbreeding
Biomass
Phylogeny
Plant Proteins
2. Zero hunger
Genetics
education.field_of_study
Vegetal Biology
Xylose
biology
Plant Stems
Full Paper
Gene silencing
food and beverages
Full Papers
Chromosomal region
Carbohydrate Metabolism
Brachypodium
RNA Interference
Xylans
Brachypodium distachyon
Coumaric Acids
Population
Quantitative Trait Loci
ComputerApplications_COMPUTERSINOTHERSYSTEMS
biomass
saccharification
Quantitative trait locus
Saccharification
Genes
Plant
Chromosomes
Plant
03 medical and health sciences
Xylan
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
Animals
Amino Acid Sequence
education
Gene
Base Sequence
Research
Glycosyltransferases
biology.organism_classification
Arabinose
030104 developmental biology
ComputingMethodologies_GENERAL
Biologie végétale
010606 plant biology & botany
Zdroj: The New Phytologist
Digital.CSIC. Repositorio Institucional del CSIC
instname
New Phytologist
New Phytologist, 2018, 218 (3), pp.974-985. ⟨10.1111/nph.15089⟩
New Phytologist 3 (218), 974-985. (2018)
New Phytologist, Wiley, 2018, 218 (3), pp.974-985. ⟨10.1111/nph.15089⟩
ISSN: 1469-8137
0028-646X
DOI: 10.1111/nph.15089⟩
Popis: Special Issue: Featured papers on ‘Plant proteases’.
The recalcitrance of secondary plant cell walls to digestion constrains biomass use for the production of sustainable bioproducts and for animal feed. We screened a population of Brachypodium recombinant inbred lines (RILs) for cell wall digestibility using commercial cellulases and detected a quantitative trait locus (QTL) associated with this trait. Examination of the chromosomal region associated with this QTL revealed a candidate gene that encodes a putative glycosyl transferase family (GT) 43 protein, orthologue of IRX14 in Arabidopsis, and hence predicted to be involved in the biosynthesis of xylan. Arabinoxylans form the major matrix polysaccharides in cell walls of grasses, such as Brachypodium. The parental lines of the RIL population carry alternative nonsynonymous polymorphisms in the BdGT43A gene, which were inherited in the RIL progeny in a manner compatible with a causative role in the variation in straw digestibility. In order to validate the implied role of our candidate gene in affecting straw digestibility, we used RNA interference to lower the expression levels of the BdGT43A gene in Brachypodium. The biomass of the silenced lines showed higher digestibility supporting a causative role of the BdGT43A gene, suggesting that it might form a good target for improving straw digestibility in crops.
C.W.'s work was funded by a Fellowship from the Burgess Foundation. Research at the CNAP was funded by The European Commission's Seventh Framework Programme (FP7), project Renewall (211982), and by BBSRC projects BB/G016178 and BB/G016194.
Databáze: OpenAIRE
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