A New High Performance Liquid Chromatography (HPLC) Method for the Quantitation of Strychnine in Urine and Tissue Extracts
| Autor: | Alois Niederwieser, R. Gitzelmann, Beat Steinmann, A. Otten, Th. Egloff, K. Pfister, W. Steiner |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Brucine
Chromatography Alkaloid Guinea Pigs education Biochemistry (medical) Clinical Biochemistry Extraction (chemistry) Glycine Strychnine General Medicine Urine High-performance liquid chromatography chemistry.chemical_compound Ammonia chemistry Child Preschool Microsomes Liver Microsome Animals Humans Child Amino Acid Metabolism Inborn Errors Chromatography High Pressure Liquid |
| Zdroj: | cclm. 20:203-206 |
| ISSN: | 1437-4331 1434-6621 |
| DOI: | 10.1515/cclm.1982.20.4.203 |
| Popis: | A high performance liquid chromatography (HPLC) method was developed for the quantitation of strychnine in urine of children with nonketotic hyperglycinaemia and other developmental disorders treated with the alkaloid. Mobile and stationary phases were polar, i.e. methanol-water-330 g/kg ammonia (volumes, 85 ml + 14.2 ml + 0.8 ml) and LiChrosorb Si-60, 7 microns. Brucine was the internal standard. Extraction was performed by the Extrelut technique. At strychnine nitrate concentrations in urine of 21, 126, and 70 micrograms/l, recovery was 92.1 +/- 8.7, 98.1 +/- 2.7, and 102.5 +/- 2.7%. A child with nonketotic hyperglycinaemia under continued strychnine treatment excreted 1 to 13.6% of the daily dose unmetabolized in urine. The method was also suitable for the estimation of unreacted strychnine in tissue extracts. The fast disappearance in vitro of strychnine from a guinea pig liver preparation was confirmed. |
| Databáze: | OpenAIRE |
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