Phospholipid molecular species distribution of oralPrevotella corporisclinical isolates
| Autor: | V. Boote, David B. Drucker, P. S. Hull, Ali M. Tavana |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Microbiology (medical)
Immunology Dental Plaque Prevotella Phospholipid Spectrometry Mass Fast Atom Bombardment Mass spectrometry Prevotella intermedia Microbiology chemistry.chemical_compound Humans Periodontal Pocket Immunology and Allergy Periodontitis Bacteroidaceae Phospholipids Phosphatidylglycerol Phosphatidylethanolamine biology Phosphatidylethanolamines Phosphatidylglycerols General Medicine Fast atom bombardment biology.organism_classification Molecular biology Infectious Diseases chemistry Biochemistry Chemotaxonomy lipids (amino acids peptides and proteins) Chromatography Thin Layer Bacteria |
| Zdroj: | FEMS Immunology & Medical Microbiology. 21:57-64 |
| ISSN: | 1574-695X 0928-8244 |
| Popis: | The aim of this study was to determine the distribution of phospholipid molecular species within Prevotella corporis of oral origin. Phospholipids of fresh clinical isolates were extracted and analysed by fast atom bombardment mass spectrometry (FAB-MS) in negative-ion mode. The major monocarboxylate anion peaks, with putative identification, observed for Prevotella corporis were m/z 241, C(15:0); 255, C(16:0); 269, C(17:1); 277, C(18:3); 279, C(18:2); 281, C(18:1). In the high mass region, major anion peaks putatively identified as individual phospholipid (PL) molecular species of Prevotella corporis were of m/z 677, PG(29:1); 691, PG(30:1); 705, PG(31:1); 706, first isotope peak of PG(31:1); and 707, PG(31:0). Related species have a different distribution of PL analogues. Separation of extracted lipid families by TLC confirmed that phosphatidylethanolamine (PE) and phosphatidylglycerol (PG) are the major polar lipids (PLs) in Prevotella corporis. Thus Prevotella corporis has a unique combination of phospholipid analogues of chemosystematic significance. |
| Databáze: | OpenAIRE |
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