Correlation of theHelicobacter pyloriadherence factor BabA with duodenal ulcer disease in four European countries
| Autor: | Markus Gerhard, Thomas Borén, Mónica Oleastro, Petra Voland, Riita Karttunen, Roland Rad, Christian Prinz, Lars Engstrand, Farzad O. Olfat, Quing Zheng |
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| Rok vydání: | 2005 |
| Předmět: |
Adult
Male Microbiology (medical) medicine.medical_specialty Genotype Spirillaceae Immunology Duodenal ulcer disease Microbiology Gastroenterology Bacterial Adhesion Helicobacter Infections Lewis Blood Group Antigens Bacterial Proteins Internal medicine medicine Humans Immunology and Allergy Adhesins Bacterial Aged Aged 80 and over Antigens Bacterial Helicobacter pylori biology Genetic Variation General Medicine Middle Aged biology.organism_classification Europe Duodenal ulcer Infectious Diseases Duodenal Ulcer Ulcer disease Female |
| Zdroj: | FEMS Immunology & Medical Microbiology. 44:151-156 |
| ISSN: | 1574-695X 0928-8244 |
| DOI: | 10.1016/j.femsim.2004.10.010 |
| Popis: | Helicobacter pylori strains harboring the vacAs1, cagA and babA2 have been associated with ulcer disease (UD). We compared the prevalence of these different genotypes and adhesive properties in H. pylori infected patients with UD in four European countries. Genomic DNA was isolated from 314 H. pylori strains: Germany (GER; n=92), Sweden (SWE, n=74), Portugal (POR, n=91) and Finland (FIN, n=57). The frequencies of babA2 genotype varied from 35% to 60%. Triple-positive strains (vacAs1+, cagA+ and babA2+) were significantly associated with UD in GER and POR and were closely correlated with UD in FIN, but not in SWE. Classification as triple-positive strains had a higher specificity for detection of UD in GER, POR and FIN than type1 or cagA+ strains. In vitro adhesion assays revealed that Swedish strains showed high adhesion properties and were thus correlated with the diagnosis of UD, although PCR detected the babA2 gene at lower frequencies and failed to show a correlation with UD. This finding appears to reflect allelic variations of the babA2 gene in SWE, although adhesive properties of the strains are retained. |
| Databáze: | OpenAIRE |
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