Pro-inflammatory cytokines increase reactive oxygen species through mitochondria and NADPH oxidase in cultured RPE cells
| Autor: | Gerd O. Till, Susan G. Elner, Zong Mei Bian, Dongli Yang, Howard R. Petty, Victor M. Elner |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Interleukin-1beta
Inflammation Pentose phosphate pathway Mitochondrion Article Proinflammatory cytokine Electron Transport Interferon-gamma Cellular and Molecular Neuroscience Onium Compounds medicine Humans Enzyme Inhibitors Pigment Epithelium of Eye Cells Cultured chemistry.chemical_classification Reactive oxygen species NADPH oxidase Dose-Response Relationship Drug biology Tumor Necrosis Factor-alpha NADPH Oxidases Hydrogen Peroxide Recombinant Proteins Sensory Systems Mitochondria Cell biology Ophthalmology Mitochondrial respiratory chain chemistry biology.protein Cytokines Tumor necrosis factor alpha Inflammation Mediators medicine.symptom Reactive Oxygen Species |
| Zdroj: | Experimental Eye Research. 85:462-472 |
| ISSN: | 0014-4835 |
| DOI: | 10.1016/j.exer.2007.06.013 |
| Popis: | Reactive oxygen species (ROS) generated during inflammation are believed to play critical roles in various ocular diseases. However, the underlying mechanisms remain poorly understood. We investigated if pro-inflammatory cytokines, tumor necrosis factor (TNF)-alpha, interleukin-1 beta (IL-1 beta), and interferon-gamma (IFN-gamma), induce ROS in human retinal pigment epithelial (RPE) cells. TNF-alpha, IL-1 beta and IFN-gamma increased both intracellular and extracellular ROS production in a time- and dose-dependent manner. Thenoyltrifluoroacetone (TTFA), an inhibitor of mitochondrial respiratory chain, blocked TNF-alpha- and IFN-gamma-, but not IL-1 beta-induced ROS, whereas other two mitochondrial respiratory chain inhibitors, rotenone and antimycin A, had no effect. NADPH oxidase inhibitor (diphenylene iodinium) abolished the ROS production induced by IL-1 beta or IFN-gamma, but not by TNF-alpha, whereas 6-aminonicotinamide (6AN), an inhibitor of the hexose monophosphate shunt (HMS), had no significant effects on the ROS induced by all three cytokines. ROS scavengers, pyrrolidinedithiocarbamate (PDTC) and N-acetyl-cysteine (NAC), reduced the levels of ROS induced by TNF-alpha, IL-1 beta and IFN-gamma (P |
| Databáze: | OpenAIRE |
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