Technology toolbox for cell line development - next generation cell line development technologies
| Autor: | Sandrine Romand, Holger Laux, Thomas Jostock, Burkhard Wilms, Ursula Bodendorf, Anett Ritter |
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| Rok vydání: | 2015 |
| Předmět: |
Engineering
Protease business.industry Chinese hamster ovary cell medicine.medical_treatment Nanotechnology General Medicine Computational biology Genome General Biochemistry Genetics and Molecular Biology law.invention Genome editing Cell culture law Poster Presentation Screening method medicine Recombinant DNA Line (text file) business |
| Zdroj: | BMC Proceedings |
| ISSN: | 1753-6561 |
| Popis: | Background Chinese hamster ovary (CHO) cells are the most widely used host for large scale production of recombinant therapeutic proteins. A combination of several gene editing approaches applied to Novartis proprietary CHO cell line resulted in a superior cell line with a significant increase of titer and improved product quality. Inter alia we have surprisingly identified a key protease responsible for proteolytic degradation of mainly non-antibody format therapeutic proteins. The recently published CHO genome in combination with screening methods and cell line engineering tools has enabled the development of this novel CHO cell line. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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