Presence of Epstein-Barr virus DNA in cerebrospinal fluid is associated with greater HIV RNA and inflammation
| Autor: | Giovanni Di Perri, Veronica Pirriatore, Gabriella Gregori, Andrea Calcagno, Sabrina Audagnotto, Daniele Imperiale, Filippo Lipani, Lorenzo Mighetto, Stefano Bonora, Valeria Ghisetti, Tommaso Lupia, Sara Gianella, Maria Grazia Milia, Cristiana Atzori |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Adult Male Herpesvirus 4 Human Immunology HIV Infections Virus Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Immune system Cerebrospinal fluid CSF pleocytosis Immunology and Allergy Medicine Humans 030212 general & internal medicine Pleocytosis Cerebrospinal Fluid business.industry Neopterin Middle Aged Viral Load medicine.disease 030104 developmental biology Infectious Diseases Cross-Sectional Studies chemistry DNA Viral Leukocytes Mononuclear RNA Female business Viral load Encephalitis |
| Zdroj: | AIDS |
| Popis: | OBJECTIVE The current study aimed to investigate whether cerebrospinal fluid (CSF) Epstein-Barr virus (EBV) or cytomegalovirus (CMV) DNA was associated with viral, inflammatory and neuronal damage biomarkers in people living with HIV (PLWH). DESIGN A cross-sectional diagnostic study on CSF fluid samples in patients undergoing lumbar punctures for clinical reasons, to better understand the role of EBV and CMV in the CNS on HIV RNA replication, blood-brain-barrier (BBB) damage and biomarkers of neuronal damage/inflammation. METHODS EBV, CMV DNA and HIV RNA were measured on CSF, through real time (RT)-PCR, from PLWHs undergoing lumbar punctures for clinical reasons (excluding oncho-haematological comorbidities). Immune-enzymatic assays evaluated blood-brain barrier inflammation and damage. Patients were stratified according to plasma HIV RNA levels in viremic (≥50 copies/ml) and aviremic ( |
| Databáze: | OpenAIRE |
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