Sister chromatid exchanges in cells defective in mismatch, post-replication and excision repair
| Autor: | Veena Afzal, Luzviminda Feeney, John P. Volpe, James E. Cleaver, Greg H. Thomas |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
DNA Replication
Genome instability Xeroderma pigmentosum DNA Repair Ultraviolet Rays DNA repair Health Toxicology and Mutagenesis Sister chromatid exchange Biology Toxicology Cell Line Caffeine Genetics medicine Postreplication repair Humans Sister chromatids Genetics (clinical) Xeroderma Pigmentosum medicine.disease Colorectal Neoplasms Hereditary Nonpolyposis Cell biology Pyrimidine Dimers DNA mismatch repair Sister Chromatid Exchange DNA Damage Nucleotide excision repair |
| Zdroj: | Mutagenesis. 10:457-462 |
| ISSN: | 1464-3804 0267-8357 |
| DOI: | 10.1093/mutage/10.5.457 |
| Popis: | Three processes associated with DNA damage and genomic instability have been defined experimentally as operating during or soon after DNA replication : mismatch repair, post-replication repair and sister chromatid exchange. All these processes appear to operate on damage and/or errors in newly replicated DNA. Both mismatch repair and post-replication repair involve resynthesis of up to 1 kb of newly synthesized DNA : mismatch repair operates on single-base or slippage errors ; post-replication repair operates on persistent gaps in newly synthesized DNA caused by damage on parental strands. Using colon cancer cells with different mismatch repair capacity, together with normal cells and excision-repair-defective and post-replication-repair-defective xeroderma pigmentosum (XP) cells, we analysed possible interactions between these processes. No evidence for overlap of mismatch repair with excision or post-replication repair was found. However, post-replication-repair-defective XP variant cells that were SV40 transformed showed higher UV-induced sister chromatid exchange frequencies than did untransformed cells. This suggests that sister chromatid exchanges in the XP variant are closely involved with UV-induced replication errors that are enhanced by transformation. |
| Databáze: | OpenAIRE |
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