Evidence for a Multiclonal Origin of Multicentric Advanced Lesions of Kaposi Sarcoma
| Autor: | Renan Duprez, Oumkaltoum Hbid, Antoine Gessain, Michel Huerre, Jean-Louis Essame Oyono, Patricia Tortevoye, Blaise Nkegoum, Marie-Jeanne Lando, Dominique Sainte-Marie, Josette Brière, Pierre Couppié, Eric Kassa-Kelembho, Céleste Lebbé, Vincent Lacoste, Camille Francès, Antoine Mahé |
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| Přispěvatelé: | Epidémiologie et Physiopathologie des Virus Oncogènes, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Service d'anatomo-pathologie [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Diderot - Paris 7 (UPD7)-Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service de Dermatologie, Centre Hospitalier Andrée Rosemon [Cayenne, Guyane Française], CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut Pasteur de Bangui, Réseau International des Instituts Pasteur (RIIP), Service de Médecine et d'Anatomopathologie, CHU de Yaoundé, Service d'Anatomopathologie, Centre Pasteur du Cameroun, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Institut Pasteur du Maroc, Institut d'Hygiène Sociale, Service de dermatologie [Paris], Université Paris Diderot - Paris 7 (UPD7)-Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Histotechnologie et Pathologie, Institut Pasteur [Paris], Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Institut Pasteur [Paris] (IP), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Université Paris Diderot - Paris 7 (UPD7)-Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (APHP), Hôpital de Cayenne, CHU Pitié-Salpêtrière [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP) |
| Rok vydání: | 2007 |
| Předmět: |
Male
Cancer Research Pathology viruses MESH: Aged 80 and over 0302 clinical medicine MESH: Child Gammaherpesvirinae Child Lymph node Skin Aged 80 and over MESH: Aged 0303 health sciences MESH: Middle Aged medicine.diagnostic_test Middle Aged Viral Load 3. Good health [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Herpesvirus 8 Human Monoclonal Female Sarcoma MESH: Viral Load Viral load Adult medicine.medical_specialty Adolescent Biology 03 medical and health sciences MESH: Skin Biopsy medicine Humans Sarcoma Kaposi Aged 030304 developmental biology MESH: Adolescent MESH: Herpesvirus 8 Human MESH: Humans MESH: Clone Cells Cancer MESH: Adult medicine.disease biology.organism_classification MESH: Male Clone Cells MESH: DNA Viral Tonsil DNA Viral MESH: Sarcoma Kaposi MESH: Female |
| Zdroj: | JNCI Journal of the National Cancer Institute JNCI Journal of the National Cancer Institute, 2007, 99 (14), pp.1086-94. ⟨10.1093/jnci/djm045⟩ JNCI: Journal of the National Cancer Institute JNCI: Journal of the National Cancer Institute, 2007, 99 (14), pp.1086-94. ⟨10.1093/jnci/djm045⟩ |
| ISSN: | 1460-2105 0027-8874 |
| DOI: | 10.1093/jnci/djm045 |
| Popis: | International audience; BACKGROUND: Kaposi sarcoma (KS) is a complex tumor of uncertain clonality. Studying the viral clonality of the human herpesvirus 8 (HHV-8) in KS to determine clonality of the tumors, a strategy that has been used previously with Epstein-Barr virus and its associated tumors, may elucidate whether multicentric (disseminated) KS lesions correspond to metastatic lesions or to expansions of independent clones. METHODS: A series of 139 KS biopsies (from skin, lymph node, or tonsil) was obtained from 98 patients, with 59 biopsies from 18 patients with disseminated multicentric KS skin lesions. The degree of spindle cell infiltration in biopsies was established by direct observation of hematoxylin-eosin-stained sections, and HHV-8 viral load was quantified by real-time polymerase chain reaction. To determine cellular clonality, the size heterogeneity of the HHV-8-fused terminal repeat (TR) region was determined by probing of electrophoresed restricted genomic DNA from KS biopsies for the HHV-8 TR sequence. RESULTS: HHV-8 clonality analysis was performed on the 62 samples for which sufficient DNA was obtained. Most samples corresponded to histologically nodular lesions with high spindle cell infiltration and high viral load. A clonal HHV-8 pattern was determined for 59 samples; 11 were found to be monoclonal and 48 to be oligoclonal. The informative samples that were from disseminated KS skin lesions (n = 26, from six patients) were either monoclonal or oligoclonal, and the size of HHV-8 episomes varied between these samples. CONCLUSION: Although some tumor KS lesions were monoclonal expansions of HHV-8-infected spindle cells, most advanced lesions were oligoclonal proliferations. Furthermore, individual KS disseminated tumor skin lesions were found to represent distinct expansions of HHV-8-infected spindle cells. Thus, our results suggest that KS lesions, especially in patients with advanced skin tumors, are reactive proliferations rather than true malignancies with metastatic dissemination. |
| Databáze: | OpenAIRE |
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