Molecular insights into ovary degeneration induced by environmental factors in female oriental river prawns Macrobrachium nipponense
| Autor: | Lifang Wang, Chunpeng Fu, Fajun Li, Tingting Li |
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| Rok vydání: | 2019 |
| Předmět: |
China
010504 meteorology & atmospheric sciences Health Toxicology and Mutagenesis Period (gene) Ovary Breeding 010501 environmental sciences Biology Toxicology 01 natural sciences Gene expression medicine Animals Thoracic ganglia Gene 0105 earth and related environmental sciences Estradiol Water Pollution General Medicine Pollution Cell biology Eyestalk medicine.anatomical_structure Female Palaemonidae Macrobrachium nipponense Environmental Monitoring Hormone |
| Zdroj: | Environmental Pollution. 253:882-888 |
| ISSN: | 0269-7491 |
| Popis: | The oriental river prawn, Macrobrachium nipponense, is an important breeding species in China. The ovary development of this prawn is regulated by the genetic factors and external environmental factors and has obvious seasonal regularity. However, the molecular mechanism of regulating ovary degradation in M. nipponense remains unclear. To address this issue, we performed transcriptome sequencing and gene expression analyses of eyestalks, cerebral ganglia (CG) and thoracic ganglia (TG) of female M. nipponense between the full ovary stage and degenerate ovary stage. Differentially expressed genes enrichment analysis results identified several important pathways such as “phototransduction-fly,” “circadian rhythm-fly” and “steroid hormone biosynthesis secretion.” In the period of ovarian degeneration, the expressions of Tim, Per2 and red pigment concentration hormone (RPCH) were significantly decreased in the eyestalk, CG and TG. And expression of 7 genes in the steroid synthesis pathway, including steryl-sulfatase, cytochrome P450 family 1 subfamily A polypeptide 1, estradiol 17β-dehydrogenase 2, glucuronosyltransferase, 3-oxo-5-alpha-steroid 4-dehydrogenase 1, estradiol 17-dehydrogenase 1 and estrone sulfotransferase was significantly decreased in the CG. Food and light signals affect the expression of clock genes and thereby decrease the expression of RPCH and the estradiol synthesis-related genes in the nervous system, which may be the main cause of ovarian degeneration in M. nipponense. The results will contribute to a better understanding of the molecular mechanisms of ovarian development regulation in crustaceans. |
| Databáze: | OpenAIRE |
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