Salivary Testosterone Determination in Studies of Child Health and Development
| Autor: | Alan Booth, Douglas A. Granger, Eve B. Schwartz, Matthew Arentz |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Adult
Male Saliva medicine.medical_specialty Serial dilution medicine.drug_class Radioimmunoassay Indicator Dilution Techniques Context (language use) Sensitivity and Specificity Behavioral Neuroscience Child Development Endocrinology Internal medicine medicine Humans Testosterone Child Detection limit Endocrine and Autonomic Systems Reproducibility of Results Testosterone (patch) Androgen Circadian Rhythm Standard curve Female Psychology Hormone |
| Zdroj: | Hormones and Behavior. 35:18-27 |
| ISSN: | 0018-506X |
| DOI: | 10.1006/hbeh.1998.1492 |
| Popis: | Measurement of hormones in children's saliva has excited interest because of numerous potential applications in developmental studies. Although assays of children's saliva for some hormones (e.g., cortisol) are widely available and used, the availability and use of assays of children's saliva testosterone is restricted. By adapting a commercially available serum testosterone kit, our laboratory has developed a reliable, efficient, and highly sensitive procedure for measuring testosterone in children's saliva that does not require separation or extraction. The minimum detection limit was 0.8 pg/mL. Intraassay coefficients of variation (CV) were between 3.66 and 6.78% at concentrations 9.25 to 86.41 pg/mL, and interassay CVs were between 5.70 and 6.61% at concentrations of 7.3 to 118.51 pg/mL. The standard curve was highly reproducible (Mslope = −0.70 andMr= 0.99). Method accuracy, determined by spike recovery, and linearity, determined by serial dilution, were 99.20 and 92.80%, respectively. Values from matched serum and saliva samples showed strong linear relationships. The assay captured near 99.09% of the range of individual differences in boys' (N= 90) and girls' (N= 85), ages 8–12, am and pm salivary testosterone levels. This assay can be easily applied to the investigation of testosterone–behavior relations in the context of studies on child health and development. It may help many child development researchers improve or expand their research activities. |
| Databáze: | OpenAIRE |
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