TopSpot® Vario: a novel microarrayer system for highly flexible and highly parallel picoliter dispensing

Autor: Peter Koltay, Roland Zengerle, Kiril Kalkandjiev, Chris Steinert
Rok vydání: 2009
Předmět:
Zdroj: Biomedical Microdevices. 11:755-761
ISSN: 1572-8781
1387-2176
DOI: 10.1007/s10544-009-9289-5
Popis: The standard TopSpot technology has been successfully used in the recent years for the highly parallel nanoliter dispensing of bio-chemical substances for microarray printing. It is based on a pneumatically actuated printhead which enables non-contact microarray fabrication at a pitch of typically 500 microm. This paper reports on a new and improved way of operating the printheads termed TopSpot Vario technology, using an incompressible material between the piezo actuator and the dispensing medium. The advantage of the incompressible medium is the direct relation between the displacement amplitude and the ejected liquid volume. Earlier reports stated that the filling of the printheads is a key issue. In this paper we report on the implementation and characterization of a new printhead design including microchannels for bubble free priming of blind channels. Microarray fabrication was successfully conducted with four different types of protein.
Databáze: OpenAIRE
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