Texturing hierarchical tissues by gradient assembling of microengineered platelet-lysates activated fibers
| Autor: | Isabel Calejo, Rui L. Reis, Rui M. A. Domingues, Manuela E. Gomes |
|---|---|
| Přispěvatelé: | Universidade do Minho |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Hierarchical tissues
Platelet lysates Science & Technology Tissue Engineering Tissue Scaffolds Stem Cells ComputingMethodologies_MISCELLANEOUS Biomedical Engineering Functionally graded scaffolds Pharmaceutical Science Bone and Bones Tendons Biomaterials ComputingMilieux_GENERAL Emulsion Electrospinning ComputingMethodologies_DOCUMENTANDTEXTPROCESSING Humans |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| Popis: | "Online ahead of print" The heterogeneity of the hierarchical tissues requires designing multipart engineered constructs as suitable tissue replacements. Herein, we propose the incorporation of platelet lysate (PL) within electrospun fiber core aiming the fabrication of functionally graded 3D scaffolds for heterotypic tissues regeneration, such as tendon-to-bone interface. First, anisotropic yarns (A-Yarns) and isotropic threads with nanohydroxyapatite (I-Threads/PL@nHAp) are fabricated to recreate the tendon- and bone- microstructures and both incorporated with PL using emulsion electrospinning for a sustained and local delivery of growth factors, cytokines, and chemokines. Biological performance using human adipose-derived stem cells demonstrates that A-Yarns/PL induce a higher expression of scleraxis, a tenogenic-marker, while in I-Threads/PL@nHAp, higher alkaline phosphatase activity and matrix mineralization suggest an osteogenic commitment without the need for external biochemical supplementation when compared to plain controls. As proof of concept, functional 3D gradient scaffolds are fabricated using weaving technique, resulting in 3D textured hierarchical constructs with gradients in composition and topography. Additionally, the precise delivery of bioactive cues together with in situ biophysical features guide the commitment into a phenotypic gradient exhibiting chondrogenic and osteochondrogenic profiles in the interface of scaffolds. Overall, we demonstrate a promising patch solution for the regeneration of tendon-to-bone tissue interface through the fabrication of PL-functional 3D gradient constructs. The authors would like to thank to Hospital da Prelada (Porto, Portugal) for the lipoaspirate tissue samples (Plastic Surgery Department). The authors acknowledge the financial support from the European Union Framework Programme for Research and Innovation HORIZON2020, Achilles (810850) and ERC CoG MagTendon (772817), and Fundacao para a Ciencia e a Tecnologia for MagTT (029930) and SmarTendon (PTDC/NAN-MAT/30595/2017) projects and the Ph.D. grant of IC (PD/BD/128088/2016). |
| Databáze: | OpenAIRE |
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