| Autor: |
Cervera, Amelia, Denisse Urbina, Peña, Marcos De La |
| Rok vydání: |
2016 |
| DOI: |
10.6084/m9.figshare.c.3612908_d9.v1 |
| Popis: |
RT-PCR amplification of negative polarity retrozyme RNAs from J. curcas and F. ananassa. A: RT-PCR experiment carried out with RNA extracts from J. curcas seeds using the direct primer Jc60D for RT to make a cDNA of the minus polarity, and the adjacent primers Jc60D and Jc60fullR (left) or Jc77D and Jc77R (right) for the PCRs (see Additional file 12). B: RT-PCR experiments performed with two different RNA extracts from F. ananassa leaves using the direct primer Fa87D for RT to make a cDNA of the minus polarity, and the adjacent primers Fa87D and Fa85R for PCR (see Additional file 12). PCR products and 100 bp DNA marker were separated by native 5 % PAGEs and stained with ethidium bromide. Controls (RT-) were the same RT-PCR experiments done without adding retrotranscriptase, whereas Controls (PCR) were PCRs done without any template. Schematic representations of a full genomic retrozyme and the positions of the oligos used for RT and PCR experiments are shown below each gel picture. (PDF 649 kb) |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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