Optimization of Vortex-Assisted Dispersive Liquid-Liquid Microextraction for the Simultaneous Quantitation of Eleven Non-Anthocyanin Polyphenols in Commercial Blueberry Using the Multi-Objective Response Surface Methodology and Desirability Function Approach
Autor: | Li Yong, Lin-Sen Qing, Yi Li, Bin Hu, Ying Xue, Xing-De Li, Shi-Hong Zhong, Xian-Shun Xu, Jing Xie |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
Liquid Phase Microextraction
Blueberry Plants Ethyl acetate Pharmaceutical Science Epigallocatechin gallate 01 natural sciences Article Analytical Chemistry Ferulic acid lcsh:QD241-441 response surface methodology chemistry.chemical_compound lcsh:Organic chemistry Limit of Detection Drug Discovery Vanillic acid Gallocatechin gallate Gallic acid Physical and Theoretical Chemistry Chromatography High Pressure Liquid blueberry Analysis of Variance Chromatography 010405 organic chemistry 010401 analytical chemistry Organic Chemistry Polyphenols Syringic acid 0104 chemical sciences vortex-assisted dispersive liquid-liquid microextraction chemistry non-anthocyanin polyphenol Chemistry (miscellaneous) Polyphenol Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Molecular Medicine desirability function approach |
Zdroj: | Molecules Volume 23 Issue 11 Molecules, Vol 23, Iss 11, p 2921 (2018) |
ISSN: | 1420-3049 |
Popis: | In the present study, 11 non-anthocyanin polyphenols, gallic acid, protocatechuate, vanillic acid, syringic acid, ferulic acid, quercetin, catechin, epicatechin, epigallocatechin gallate, gallocatechin gallate and epicatechin gallate&mdash were firstly screened and identified from blueberries using an ultra performance liquid chromatography&ndash time of flight mass spectrography (UPLC-TOF/MS) method. Then, a sample preparation method was developed based on vortex-assisted dispersive liquid-liquid microextraction. The microextraction conditions, including the amount of ethyl acetate, the amount of acetonitrile and the solution pH, were optimized through the multi-objective response surface methodology and desirability function approach. Finally, an ultra performance liquid chromatography&ndash triple quadrupole mass spectrography (UPLC-QqQ/MS) method was developed to determine the 11 non-anthocyanin polyphenols in 25 commercial blueberry samples from Sichuan province and Chongqing city. The results show that this new method with high accuracy, good precision and simple operation characteristics, can be used to determine non-anthocyanin polyphenols in blueberries and is expected to be used in the analysis of other fruits and vegetables. |
Databáze: | OpenAIRE |
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