Laboratory colonization of Anopheles pseudopunctipennis (Diptera: Culicidae) without forced mating
| Autor: | Vicente Quispe, Bernard Bouchité, Roberto Rodríguez, Libia Torrez, Frédéric Lardeux, Tamara Chavez, Rosenka Tejerina |
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| Přispěvatelé: | Institut de Recherche pour le Développement - IRD Bolivie, Instituto Nacional de Laboratorios de Salud, La Paz, Bolivia (INLASA), Escuela Tecnica de Salud - Cochabamba |
| Rok vydání: | 2007 |
| Předmět: |
Male
forced mating Oviposition 030231 tropical medicine Biology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Sexual Behavior Animal 0302 clinical medicine Animals Laboratory Anopheles Animals Colonization Mating Protozoal disease Malaria vector natural mating 030304 developmental biology Blue light 0303 health sciences insectary General Immunology and Microbiology Ecology anopheles pseudopunctipennis General Medicine colonization 3. Good health [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology Anopheles pseudopunctipennis Larva Female [SDE.BE]Environmental Sciences/Biodiversity and Ecology General Agricultural and Biological Sciences |
| Zdroj: | Comptes Rendus Biologies Comptes Rendus Biologies, Elsevier, 2007, 330 (8), pp.571-575. ⟨10.1016/j.crvi.2007.04.002⟩ |
| ISSN: | 1631-0691 |
| Popis: | International audience; Anopheles pseudopunctipennis is one of the main malaria vectors in the Andean regions of South America. Few experimental data exist on this species because it is not very available in laboratories due to its eurygamic status that makes colony maintenance difficult. Indeed, individuals do not mate in the confined space of insectary cages. To avoid this problem, forced artificial mating can be used. However, this technique is time consuming, requires a well-trained technician, and is inadequate for easy mass production, which is sometimes necessary for certain experimental works. This study presents a technique based on exposure of adult mosquitoes to a blue stroboscopic light for 20 min during several nights, which encourages them to copulate naturally under laboratory conditions. After some generations, a self-free-mating strain was obtained. The technique is simple, inexpensive and is probably effective whatever the An. pseudopunctipennis strain considered. |
| Databáze: | OpenAIRE |
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