Coxiella burnetii serology assays in goat abortion storm
| Autor: | Mohamed Ait Ichou, Eileen N. Ostlund, Michelle P. Emery, David McFarling, Luanne McGonigle, Jeff Ballin |
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| Rok vydání: | 2014 |
| Předmět: |
DNA
Bacterial Q fever Enzyme-Linked Immunosorbent Assay Abortion Biology Tick Polymerase Chain Reaction Sensitivity and Specificity Serology law.invention Disease Outbreaks Antigen law Pregnancy medicine Animals Polymerase chain reaction Goat Diseases General Veterinary Goats Complement Fixation Tests Abortion Veterinary Complement fixation test Coxiella burnetii biology.organism_classification medicine.disease Virology Antibodies Bacterial United States Female Q Fever |
| Zdroj: | Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 26(1) |
| ISSN: | 1943-4936 |
| Popis: | Many commercial antibody detection enzyme-linked immunosorbent assay (ELISA) kits for Q fever utilize the Nine Mile (Montana tick) strain of Coxiella burnetii as antigen. An ELISA kit manufactured in France employs ovine placenta-sourced antigen and has been used in Europe. Sera from goats experiencing a Q fever abortion storm in the United States were used to compare the sensitivity and specificity of these 2 ELISA formats and the Q fever complement fixation test (CFT). Latent class estimates of sensitivity ranged from 97% to 100% with a specificity of 95–100% for the 2 ELISA kits. Estimates for sensitivity and specificity of the CFT were 89% and 82%, respectively. There was not a significant increase in ELISA sensitivity observed with the ovine-sourced antigen kit in this study. Real-time polymerase chain reactions performed on a portion of the sera found that 15 out of 20 sera were congruent across 4 tests for positive and negative sera. |
| Databáze: | OpenAIRE |
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