Binding of the B cell activation antigen B7 to CD28 costimulates T cell proliferation and interleukin 2 mRNA accumulation
Autor: | Peter S. Linsley, Laura Sue Grosmaire, Jeffrey A. Ledbetter, Nitin K. Damle, Alejandro Aruffo, William E. Brady |
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Rok vydání: | 1991 |
Předmět: |
Antigens
Differentiation T-Lymphocyte Transcription Genetic Recombinant Fusion Proteins T-Lymphocytes T cell Molecular Sequence Data Immunology chemical and pharmacologic phenomena Biology Lymphocyte Activation Transfection Polymerase Chain Reaction Cell Line Interleukin 21 CD28 Antigens medicine Animals Humans Immunology and Allergy Cytotoxic T cell RNA Messenger IL-2 receptor Antigen-presenting cell Interleukin 3 Base Sequence ZAP70 CD28 hemic and immune systems Articles Molecular biology Receptors Interleukin-4 Kinetics medicine.anatomical_structure Receptors Mitogen Interleukin-2 Interleukin-4 Oligonucleotide Probes Plasmids |
Zdroj: | The Journal of Experimental Medicine |
ISSN: | 1540-9538 0022-1007 |
Popis: | A successful immune response requires intercellular contact between T and B lymphocytes. We recently showed that CD28, a T cell surface protein that regulates an activation pathway, could mediate intercellular adhesion with activated B cells by interaction with the B7 antigen. Here we show that CD28 is the primary receptor for B7 on activated peripheral blood T cells, that CD28 binds to B7 in the absence of other accessory molecules, and that interaction between CD28 and B7 is costimulatory for T cell activation. To characterize the binding of CD28 to B7, we have produced genetic fusions of the extracellular portions of B7 and CD28, and immunoglobulin (Ig) C gamma 1 chains. 125I-labeled B7 Ig bound to CD28-transfected Chinese hamster ovary (CHO) cells, and to immobilized CD28 Ig with a Kd approximately 200 nM. B7 Ig also inhibited CD28-mediated cellular adhesion. The function of CD28-B7 interactions during T cell activation was investigated with soluble fusion proteins and with B7-transfected CHO cells. Immobilized B7 Ig and B7+ CHO cells costimulated T cell proliferation. Stimulation of T cells with B7+ CHO cells also specifically increased levels of interleukin 2 transcripts. These results demonstrate that the CD28 signaling pathway could be activated by B7, resulting in increased T cell cytokine production and T cell proliferation. Cellular interactions mediated by B7 and CD28 may represent an important component of the functional interactions between T and B lymphoid cells. |
Databáze: | OpenAIRE |
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