Streptomyces aureorectus DSM 41692 and Streptomyces virens DSM 41465 are producers of the antibiotic nucleocidin and 4'-fluoroadenosine is identified as a co-product
| Autor: | Xuan Feng, Yawen Chen, Marta Wojnowska, Qingzhi Zhang, David O'Hagan |
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| Přispěvatelé: | European Commission, University of St Andrews. School of Chemistry, University of St Andrews. Biomedical Sciences Research Complex, University of St Andrews. EaSTCHEM |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Natural product Chemistry Metabolite Organic Chemistry Streptomyces aureorectus NDAS QD Chemistry Biochemistry Homology (biology) AC Microbiology chemistry.chemical_compound Enzyme Streptomyces calvus Gene cluster Streptomyces virens QD Physical and Theoretical Chemistry |
| Popis: | Authors thank the Leverhulme Trust, EPSRC and the Sinfonia consortium of the EU for grants as well as the Chinese Scholarship Council for a Studentship (YC), all of which helped to support this work. Genome homology and the presence of a putative biosynthetic gene cluster identified Streptomyces aureorectus DSM 41692 and Streptomyces virens DSM 41465 as candidate producers of the antibiotic nucleocidin 1. Indeed when these bacterial strains were cultured in a medium supplemented with fluoride (4 mM) they each produced nucleocidin 1 and the previously identified 4'-fluoro-3'-O-β-glucosylated adenosine 2 and its sulfamylated derivative 3. In both of these cases 4'-fluoroadenosine 9 is also identified as a natural product although it has never been observed during fermentations of Streptomyces calvus, the original source of nucleocidin 1. The identity of 4'-fluoroadenosine 9 was confirmed by a total synthesis as well as by its in vitro enzymatic conversion to metabolite 2 using the glucosyl transferase enzyme, NucGT. Postprint Postprint |
| Databáze: | OpenAIRE |
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