Separation and characterization of sucrose esters from Oriental tobacco leaves using accelerated solvent extraction followed by SPE coupled to HPLC with ion-trap MS detection
| Autor: | Xiaolan Zhu, Guangming Huang, Tu Yonggao, Jun Yang, Zhang-Hai Li, Hongying Zhu, Pan Wenjie, Feng Yonggang |
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| Rok vydání: | 2013 |
| Předmět: |
Electrospray
Sucrose Chromatography Chemistry Extraction (chemistry) Solid Phase Extraction Filtration and Separation Esters High-performance liquid chromatography Mass Spectrometry Analytical Chemistry Plant Leaves Cartridge chemistry.chemical_compound Accelerated solvent extraction Liquid chromatography–mass spectrometry Tobacco Solvents Ion trap Chromatography High Pressure Liquid |
| Zdroj: | Journal of separation science. 36(15) |
| ISSN: | 1615-9314 |
| Popis: | Sucrose esters (SEs) were successfully extracted from Oriental tobacco leaves using a new methodology based on accelerated solvent extraction followed by hydrophilic–lipophilic balanced cartridge cleanup step. The SEs were detected by HPLC with ion-trap MS detection using an electrospray interface operated in the positive ion mode. This methodology combines the high efficiency of extraction provided by a pressurized fluid and the highly sensitive characterization offered by ion-trap MS. Under the optimized conditions, 14 SEs were first identified among a total of 23 SEs found in Oriental tobacco leaves. Under the same conditions, only four new SEs were extracted by using traditional ultrasound-assisted extraction and liquid–solid extraction methods. The present method might be potentially useful in high-efficiency extraction and sensitive characterization of SEs from complex matrices such as tobacco leaves. |
| Databáze: | OpenAIRE |
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