Serotype determination of adenoviruses in children with respiratory infection
| Autor: | Ahmad Nejati, Kambiz Ahmadi, Alireza Samarbafzadeh, Saeed Najafifard, Niloofar Neisi, Mojtaba Rasti, Yusuf Ghasemi, Roya Nikfar, Ahmad Shamsizadeh, Manoochehr Makvandi |
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| Rok vydání: | 2013 |
| Předmět: |
Serotype
Male Adolescent viruses medicine.disease_cause Microbiology law.invention Adenoviridae law Lower respiratory tract infection Medicine Humans Serotyping Child Respiratory Tract Infections Polymerase chain reaction Respiratory tract infections business.industry Respiratory infection Infant medicine.disease Virology medicine.anatomical_structure Cross-Sectional Studies Child Preschool Pediatrics Perinatology and Child Health Acute Disease Female business Nested polymerase chain reaction Respiratory tract |
| Zdroj: | Indian journal of pediatrics. 81(7) |
| ISSN: | 0973-7693 |
| Popis: | To determine adenovirus serotypes among children with acute respiratory infection (ARI) in Khoozestan province, Iran during years 2010–2011. One hundred sixty three nasopharyngeal swabs were collected from children between 1 and 15 y who were hospitalized for the acute respiratory infection. The viral DNA was extracted from the nasopharyngeal swabs and adenoviruses were detected by Nested PCR. Positive PCR samples were sequenced in order to confirm the adenovirus serotypes. Out of 163 samples, 30 (18.4 %) were positive for adenoviruses by nested PCR. Twenty two PCR products were sequenced and recognized as Ad5 and Ad2 serotypes including 19 (86.3 %) Ad5 and 3 (13.7 %) Ad2. This study reveals that adenoviruses with Ad5 predominance are important cause of respiratory tract infection in children. |
| Databáze: | OpenAIRE |
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