Understanding flow dynamics, viability and metastatic potency of cervical cancer (HeLa) cells through constricted microchannel
| Autor: | Amaresh Dalal, Gautam Biswas, Asif Raza, Binita Nath, Siddhartha Sankar Ghosh, Vishal Sethi |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cell Survival Population Microfluidics lcsh:Medicine Uterine Cervical Neoplasms Apoptosis Article HeLa 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine In vivo Cell Line Tumor Ethidium Humans Neoplasm Metastasis lcsh:Science education education.field_of_study Multidisciplinary Microchannel biology lcsh:R Acridine orange Microfluidic Analytical Techniques biology.organism_classification Acridine Orange 030104 developmental biology chemistry Cell culture 030220 oncology & carcinogenesis Biophysics lcsh:Q Female Ethidium bromide HeLa Cells |
| Zdroj: | Scientific Reports Scientific Reports, Vol 8, Iss 1, Pp 1-10 (2018) |
| ISSN: | 2045-2322 |
| Popis: | To understand the burgeoning challenges of metastasis, a microchannel of 35 μm diameter, constricted to 7 μm for a distance of 200 μm in a total length of 3 mm, was designed and fabricated using a mask aligner made of polydimethylsiloxane (PDMS) to mimic in vivo capillaries. A thin glass cover-slide was mounted on top to monitor the motion of single or aggregated malignant HeLa cells (size 17–30 μm) microscopically through the constricted microchannel at a constant flow rate of 30 μl/h. Quantitative deconvolution of high-speed videographs of a single cell of 30 μm revealed cellular deformation while passing through constriction, having elongation index, average transit velocity and entry time of 2.67, 18 mm/s and 5.1 ms, respectively. Morphological analysis of live and apoptotic cells by dual staining with Acridine Orange/Ethidium Bromide demonstrated retention of a significant viable cell population after exit through the constriction and a viability index of 50% was quantified by dye exclusion assay. The cumulative data for microfluidic parameters, morphology and relevant metastatic MMP2 gene expression efficiency measured by real-time polymerase chain reaction revealed retention of virulence potency that could possibly cause metastasis, would be beneficial in developing futuristic MEMS device for cancer theranostics. |
| Databáze: | OpenAIRE |
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