Ultrabright and Fluorogenic Probes for Multicolor Imaging and Tracking of Lipid Droplets in Cells and Tissues
| Autor: | Tkhe Kyong Fam, Thierry Galli, Andrey S. Klymchenko, Pichandi Ashokkumar, Lydia Danglot, Mayeul Collot, Orestis Faklaris |
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| Přispěvatelé: | Laboratoire de Bioimagerie et Pathologies (LBP), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Biophotonique et Pharmacologie (CNRS UMR 7213), Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biophotonique et Pharmacologie - UMR 7213 (LBP), Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA)) |
| Rok vydání: | 2018 |
| Předmět: |
Models
Molecular [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] Indoles multicolor imaging 02 engineering and technology 010402 general chemistry 01 natural sciences Biochemistry Catalysis KB Cells chemistry.chemical_compound Mice Colloid and Surface Chemistry Lipid droplet Microscopy Organelle Moiety Animals Humans Merocyanine Benzopyrans Fluorescent Dyes tissue imaging communication Chemistry Optical Imaging Nile red dioxaborine General Chemistry Lipid Droplets 021001 nanoscience & nanotechnology Fluorescence Two-Photon Excitation imaging 0104 chemical sciences Mice Inbred C57BL Adipose Tissue Liver Microscopy Fluorescence Biophysics 0210 nano-technology Intracellular |
| Zdroj: | Journal of the American Chemical Society Journal of the American Chemical Society, American Chemical Society, 2018, 140 (16), ⟨10.1021/jacs.7b12817⟩ |
| ISSN: | 1520-5126 0002-7863 |
| DOI: | 10.1021/jacs.7b12817⟩ |
| Popis: | Lipid droplets (LDs) are intracellular lipid-rich organelles that regulate the storage of neutral lipids and were recently found to be involved in many physiological processes, metabolic disorders, and diseases including obesity, diabetes, and cancers. Herein we present a family of new fluorogenic merocyanine fluorophores based on an indolenine moiety and a dioxaborine barbiturate derivative. These so-called StatoMerocyanines (SMCy) fluoresce from yellow to the near-infrared (NIR) in oil with an impressive fluorescence enhancement compared to aqueous media. Additionally, SMCy display remarkably high molar extinction coefficients (up to 390 000 M–1 cm–1) and high quantum yield values (up to 100%). All the members of this new family specifically stain the LDs in live cells with very low background noise. Unlike Nile Red, a well-known lipid droplet marker, SMCy dyes possess narrow absorption and emission bands in the visible, thus allowing multicolor imaging. SMCy proved to be compatible with fixation and le... |
| Databáze: | OpenAIRE |
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