miR-618 Suppresses Metastasis in Gastric Cancer by Downregulating the Expression of TGF-β2
| Autor: | Lijie Gong, Zhenye Lv, Jungang Luo, Hou-Quan Tao, Zaiyuan Ye, Legao Chen, Ji Xu, Ying-Jie Xia, Ji Shi, Guangyuan Song |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Male Histology Down-Regulation Apoptosis In situ hybridization Biology Metastasis 03 medical and health sciences Transforming Growth Factor beta2 0302 clinical medicine Western blot Cell Movement Stomach Neoplasms Cell Line Tumor microRNA Gene expression medicine Humans Genes Tumor Suppressor Neoplasm Invasiveness Ecology Evolution Behavior and Systematics Aged Cell Proliferation medicine.diagnostic_test Cell Cycle Stomach Cancer Cell cycle Middle Aged medicine.disease Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology Real-time polymerase chain reaction Cancer research Female Anatomy 030217 neurology & neurosurgery Biotechnology Signal Transduction |
| Zdroj: | Anatomical record (Hoboken, N.J. : 2007). 302(6) |
| ISSN: | 1932-8494 |
| Popis: | Recent studies have demonstrated that microRNAs regulate gene expression and are related to cancer progression. Increasing evidence shows that miR-618 plays an important role in a variety of tumors, including thyroid carcinomas, breast cancer and lymphoma cancer. However, no studies have examined the expression or function of miR-618 in gastric cancer (GC). In this study, we examined the effects and molecular mechanisms of miR-618 in GC. We compared the expression levels of miR-618 in 90 paired GC tissues and adjacent noncancerous tissues. Cell cycle, apoptosis and transwell assays were performed in GC cells with miR-618 mimic or inhibitor in vitro. We first used quantitative PCR(qPCR) to show that miR-618 expression levels were downregulated in GC tissues, which showed statistical significance. Next we used transwell assays to prove that miR-618 suppressed the invasion and migration capacity of GC cells. Furthermore, screening of the miRDB and Target Scan Human databases indicated TGF-β2 as a downstream target of miR-618. In further research, we identified TGF-β2 as a target gene of miR-618 by the luciferase reporter assay. Western blot analysis confirmed that TGF-β2 expression was inversely correlated with miR-618 expression. In situ hybridization showed that miR-618 expression level was downregulated in GC tissues. In conclusion, our findings suggest that miR-618 may function as a tumor suppressor in GC and suppresses metastasis in GC by negatively regulating the transcriptional level of TGF-β2. Anat Rec, 302:931-940, 2019. © 2019 Wiley Periodicals, Inc. |
| Databáze: | OpenAIRE |
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