Different expression of LHR, PRLR, GH and IGF1 during testicular development of yak
| Autor: | Fulong Ma, Yin Mancai, Yan Ping, Chunnian Liang, Xian Guo, Yongfu La, Xiaoming Ma, Min Chu, Li Jiye, Bao Pengjia |
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| Rok vydání: | 2021 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Receptors Prolactin medicine.medical_treatment Spleen Biology Endocrinology Internal medicine Testis Gene expression medicine Animals Insulin-Like Growth Factor I Leydig cell urogenital system Prolactin receptor Growth factor luteinizing hormone/choriogonadotropin receptor Leydig Cells Skeletal muscle Receptors LH medicine.anatomical_structure Growth Hormone Cattle Animal Science and Zoology hormones hormone substitutes and hormone antagonists Germ cell Biotechnology |
| Zdroj: | Reproduction in Domestic Animals. 57:221-227 |
| ISSN: | 1439-0531 0936-6768 |
| DOI: | 10.1111/rda.14044 |
| Popis: | Luteinizing hormone receptor (LHR), prolactin receptor (PRLR), growth hormone (GH) and insulin-like growth factor 1 (IGF1) have been shown to be key regulators of germ cell development. However, the role of LHR, PRLR, GH and IGF1 in the development of yak testis remains unclear. In this study, we aimed to describe and compare gene expression and protein localization of LHR, PRLR, GH and IGF1 in the development of yak testes. Testes were collected from 6, 24, 36 and 72 months yak, and the kidney, liver, testicular, lung, skeletal muscle, heart and spleen tissues were collected from 36 months yak. The quantitative real-time PCR (qRT-PCR) results showed that the expression of these four genes was widely expressed in kidney, liver, testicular, lung, skeletal muscle, heart and spleen, while the LHR and PRLR were highly expressed in the kidney, skeletal muscle and testis, and higher levels of GH and IGF were expressed in spleen and testis. Moreover, the mRNA expression of these genes in adults was higher than in pre-pubertal yak. In the testis, the LHR-, PRLR-, GH- and IGF1-positive signals were detected in the Leydig cells of the 6 months, while the intense positive signals were discovered in Leydig cells, spermatogonia and spermatocytes of the 36 and 72 months. Thus, LHR, PRLR, GH and IGF1 may be involved in the development of spermatids and spermatocytes, and in the regulation of spermatogonia proliferation and Leydig cell function. |
| Databáze: | OpenAIRE |
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