Bioconjugation of Gold Nanoparticles through the Oxidative Coupling of ortho-Aminophenols and Anilines
| Autor: | Stacy L. Capehart, Adel M. ElSohly, Allie C. Obermeyer, Matthew B. Francis |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Potassium
Oligonucleotides Biomedical Engineering Pharmaceutical Science chemistry.chemical_element Bioengineering Aminophenols Adsorption Organic chemistry Ferricyanides Pharmacology chemistry.chemical_classification Aniline Compounds Aqueous solution Bioconjugation Oxidative Coupling Biomolecule Organic Chemistry Proteins Combinatorial chemistry chemistry Colloidal gold Reagent Oxidative coupling of methane Gold Peptides Biotechnology |
| Zdroj: | Bioconjugate Chemistry. 25:1888-1892 |
| ISSN: | 1520-4812 1043-1802 |
| DOI: | 10.1021/bc5003746 |
| Popis: | While there are a number of methods for attaching gold nanoparticles (AuNPs) to biomolecules, the existing strategies suffer from nonspecific AuNP adsorption, reagents that are unstable in aqueous solutions, and/or long reaction times. To improve upon existing AuNP bioconjugation strategies, we have adapted a recently reported potassium ferricyanide-mediated oxidative coupling reaction for the attachment of aniline-functionalized AuNPs to o-aminophenol-containing oligonucleotides, peptides, and proteins. The aniline-AuNPs are stable in aqueous solutions, show little-to-no nonspecific adsorption with biomolecules, and react rapidly (30 min) with o-aminophenols under mild conditions (pH 6.5, 1 mM oxidant). |
| Databáze: | OpenAIRE |
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