Stepwise occlusion of the carotid arteries of the rat: MRI assessment of the effect of donepezil and hypoperfusion-induced brain atrophy and white matter microstructural changes

Autor: Zsófia Spisák, András Czurkó, Gabriella Nyitrai, Dávid Gajári, Pálma Diószegi, Tamás Z. Kincses, Tamás Spisák
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Central Nervous System
Male
lcsh:Medicine
Pathology and Laboratory Medicine
Nervous System
Diagnostic Radiology
Brain Ischemia
0302 clinical medicine
Piperidines
Materials Physics
Functional Magnetic Resonance Imaging
Occlusion
Medicine and Health Sciences
Carotid Stenosis
Donepezil
Common carotid artery
lcsh:Science
Microstructure
Brain Mapping
Multidisciplinary
medicine.diagnostic_test
Radiology and Imaging
Physics
Brain
Magnetic Resonance Imaging
White Matter
medicine.anatomical_structure
Diffusion Tensor Imaging
Cerebral blood flow
Cerebrovascular Circulation
Physical Sciences
Indans
Cardiology
Anatomy
medicine.drug
Research Article
medicine.medical_specialty
Imaging Techniques
Carotid Artery
Common
Brain Morphometry
Materials Science
Neuroimaging
Research and Analysis Methods
White matter
03 medical and health sciences
Signs and Symptoms
Diagnostic Medicine
Internal medicine
medicine.artery
mental disorders
medicine
Animals
Rats
Wistar
business.industry
lcsh:R
Biology and Life Sciences
Magnetic resonance imaging
Voxel-based morphometry
Rats
Neostriatum
Oxygen
030104 developmental biology
lcsh:Q
Atrophy
business
030217 neurology & neurosurgery
Diffusion MRI
Neuroscience
Zdroj: PLoS ONE
PLoS ONE, Vol 13, Iss 5, p e0198265 (2018)
ISSN: 1932-6203
Popis: Bilateral common carotid artery occlusion (BCCAo) in the rat is a widely used animal model of vascular dementia and a valuable tool for preclinical pharmacological drug testing, although the varying degrees of acute focal ischemic lesions it induces could interfere with its translational value. Recently, a modification to the BCCAo model, the stepwise occlusion of the two carotid arteries, has been introduced. To acquire objective translatable measures, we used longitudinal multimodal magnetic resonance imaging (MRI) to assess the effects of semi-chronic (8 days) donepezil treatment in this model, with half of the Wistar rats receiving the treatment one week after the stepwise BCCAo. With an ultrahigh field MRI, we measured high-resolution anatomy, diffusion tensor imaging, cerebral blood flow measurements and functional MRI in response to whisker stimulation, to evaluate both the structural and functional effects of the donepezil treatment and stepwise BCCAo up to 5 weeks post-occlusion. While no large ischemic lesions were detected, atrophy in the striatum and in the neocortex, along with widespread white matter microstructural changes, were found. Donepezil ameliorated the transient drop in the somatosensory BOLD response in distant cortical areas, as detected 2 weeks after the occlusion but the drug had no effect on the long term structural changes. Our results demonstrate a measurable functional MRI effect of the donepezil treatment and the importance of diffusion MRI and voxel based morphometry (VBM) analysis in the translational evaluation of the rat BCCAo model.
Databáze: OpenAIRE
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