Th2 immune response by the iron-regulated protein HupB of Mycobacterium tuberculosis
| Autor: | Jahnavi Virivinti, Manjula Sritharan, Subhakar Kandi, Mitali Choudhury, Venkataraman Sritharan |
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| Rok vydání: | 2020 |
| Předmět: |
Tuberculosis
Iron Mutant Peripheral blood mononuclear cell Microbiology Mycobacterium tuberculosis 03 medical and health sciences Interferon-gamma Mice Immune system medicine Animals Humans Tuberculosis Pulmonary 0303 health sciences biology 030306 microbiology business.industry Wild type Immunity biology.organism_classification medicine.disease Infectious Diseases Cell culture biology.protein Antibody business |
| Zdroj: | The Indian journal of tuberculosis. 69(1) |
| ISSN: | 0019-5707 |
| Popis: | Background HupB is an iron-regulated protein essential for the growth of Mycobacterium tuberculosis inside macrophages. To investigate if HupB induced a dominant Th2 type immune response, we studied the effect of rHupB on PBMCs from TB patients and by infecting mouse macrophages with wild type and hupB KO mutants. Methods PBMCs from pulmonary TB (n = 60), extra pulmonary TB (n = 23) and healthy controls (n = 30) were stimulated with purified HupB and the cytokines secreted were assayed. The sera were screened for anti-HupB antibodies by ELISA. Mouse macrophages cell line (RAW 264.7) was infected with wild type, hupB KO and hupB-complemented strains of M. tuberculosis grown in high and low iron medium and the expression of cytokines was assayed by qRT-PCR. Results Murine macrophages infected with the hupB KO strain produced low levels of the pro-inflammatory cytokines IFN-γ, TNF-α, IL-1, and IL-18 and high levels of IL-10. HupB induced IL-6 and IL-10 production in PBMCs of TB patients and down-regulated IFN-γ and TNF-α production. The influence of HupB was remarkable in the EPTB group. Conclusion HupB shifted the immune response to the Th2 type. Low IFN-γ and elevated IL-10 in EPTB patients is noteworthy. |
| Databáze: | OpenAIRE |
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