Formation of ethyl acetate by Kluyveromyces marxianus on whey: studies of the ester stripping
| Autor: | Thanet Urit, Christian Löser, Martin Wunderlich, Thomas Bley |
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| Rok vydání: | 2010 |
| Předmět: |
Stripping (chemistry)
Ethyl acetate Lactose Bioengineering Acetates Industrial Microbiology Kluyveromyces chemistry.chemical_compound Bioreactors Kluyveromyces marxianus Bioreactor Chromatography biology General Medicine biology.organism_classification Aerobiosis Culture Media Trace Elements Models Chemical chemistry Yield (chemistry) Fermentation Dairy Products Aeration Biotechnology |
| Zdroj: | Bioprocess and Biosystems Engineering. 34:547-559 |
| ISSN: | 1615-7605 1615-7591 |
| DOI: | 10.1007/s00449-010-0504-9 |
| Popis: | Kluyveromyces marxianus is capable of converting lactose into ethyl acetate offering a chance for an economical reuse of whey. The microbial formation of ethyl acetate as a bulk product calls for an aerobic process and, thus, the highly volatile ethyl acetate is discharged from the aerated bioreactor. This stripping process was modeled and investigated experimentally. The stripping rate was proportional to the gas flow and nearly independent of the stirring rate since the stripping was governed by the absorption capacity of the exhaust gas rather than the phase transfer. Cooling the exhaust gas did not noticeably influence the stripping. One batch experiment is presented in detail to demonstrate the formation of ethyl acetate by K. maxianus DSM 5422 on whey. Further batch experiments showed that a substantial formation of ethyl acetate only occurred when the yeast growth was limited by a lack of trace elements. The highest product yield observed was 0.25 g ethyl acetate per g lactose which is nearly 50% of the theoretical maximum. |
| Databáze: | OpenAIRE |
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