Effect of inner pH on peptide acylation within PLGA microspheres
| Autor: | Kuntang Liu, Zhengjie Meng, Hao Ren, He Huang, Zhe Liu, Jiwei Liu, Yan Xu, Yonglu Wang, Xueming Li |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Polymers
Acylation Octreotide acetate Pharmaceutical Science Peptide 02 engineering and technology 030226 pharmacology & pharmacy Oligomer 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Polymer degradation Drug Delivery Systems Drug Development Polylactic Acid-Polyglycolic Acid Copolymer Polymer chemistry medicine Aminoacylation chemistry.chemical_classification Lactide Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology Microspheres PLGA Drug Liberation Kinetics chemistry lipids (amino acids peptides and proteins) Swelling medicine.symptom 0210 nano-technology |
| Zdroj: | European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences. 134 |
| ISSN: | 1879-0720 |
| Popis: | Polymer degradation within the controlled-release depots comprising of lactide and glycolide (PLGA) forms an acidic microenvironment, in which severe acylation of the peptide by the polymer degradation products takes place. The aim of this study was to make out the role of the inner μpH on peptide acylation within the microspheres and how could it influence the reaction. The effects of pH on the acylation reaction within microspheres were composed of two aspects. Firstly, the inherent effect of pH on the acylation reaction itself was figured out: with the pH environment going up from acid to neutral, a model peptide (octreotide acetate) acylation became more and more serious. Then, the multivariate effect of pH on the dynamic microsphere delivery system especially the state of the acylation substrates (drug and oligomer) was investigated. When the inner pH was neutralized by Ca(OH)2 to varying degrees, polymer degradation rate, drug release rate, polymer degradation mechanism and oligomer accumulation state within the microspheres all changed. These changes highly affected the mass transfer of the acylation substrates to the external release medium. Neutralization of the μpH prolonged the retention time of drug and oligomer within the microspheres. Water absorption and single microsphere swelling experiments all showed a higher retention amount of acylation substrates during the critical period for peptide acylation. Generally, when the inner μpH was neutralized, except that the neutral environment itself promoted acylation reaction, the effects of pH on the dynamic system were also highly responsible for the serious acylation within the microspheres. |
| Databáze: | OpenAIRE |
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