The Effect of Laser Photobiomodulation on Periodontal Ligament Stem Cells

Autor: Kaveh Baghaei, Gilda Parsamanesh, Reza Fekrazad, Leila Gholami, Shiva Shahabi, Marzieh Jazaeri
Rok vydání: 2020
Předmět:
Zdroj: Photochemistry and Photobiology.
ISSN: 1751-1097
0031-8655
Popis: Photobiomodulation (PBM) is considered as a non-invasive procedure with the potential of inducing favorable changes in cellular behavior. In this study, we aimed to evaluate the effects of near infra-red low-intensity laser PBM on proliferation, viability, and osteogenic differentiation of stem cells isolated from human periodontal ligament. A 940 nm diode laser with an energy density of 4 J/cm2 in a 100-mW continuous wave was used for irradiation in 3 sessions every 48h. Cell viability was measured 24, 48, and 72 hours after irradiation. The effects of laser on mineralized tissue deposition were evaluated by using Alizarin red staining after dividing cells into three groups of non-osteogenic medium (C-), an osteogenic medium without laser (C+), and an osteogenic medium with laser irradiation (L+). Gene expression levels were also evaluated by Real-Time PCR. Our results showed no significant difference between MTT levels of the study and control groups. After 14 and 21 days, both L+ and C+ groups showed an increase in mineralized tissue formation compared to the C- group. There was an increase in VEGF and BMP expressions compared to C-. In conclusion, the irradiation setting used in this study may be able to improve mineralized tissue deposition.
Databáze: OpenAIRE
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