Nitrogen depletion in Arthrospira sp. PCC 8005, an ultrastructural point of view
| Autor: | Juan Carlos Cabrera Pino, Ruddy Wattiez, Raphaël Facchini, Patrick Flammang, Frédéric Deschoenmaeker, Guillaume Bayon-Vicente, Neha Sachdeva |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cyanobacteria Nitrogen 030106 microbiology chemistry.chemical_element 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins Structural Biology Spirulina Photosynthesis Glycogen biology Nitrogen deficiency Gene Expression Regulation Bacterial biology.organism_classification Carbon 030104 developmental biology chemistry Biochemistry Starvation Thylakoid Ultrastructure Phycobilisome |
| Zdroj: | Journal of structural biology. 196(3) |
| ISSN: | 1095-8657 |
| Popis: | In cyanobacteria, the nitrogen and carbon metabolisms are functionally bridged and consequently respond to the carbon-to-nitrogen ratio. Consequently, a nitrogen deficiency results in carbon excess. For the first time, the biological adaptation of Arthrospira sp. PCC 8005 to nitrogen starvation has been deeply characterized at the cellular structure scale. The results indicated that the carbon excess is rerouted into carbon storage granules, such as the polyhydroxyalkanoate and glycogen granules corroborating existing data. Additionally, this photosynthetic organism hugely secreted exopolysaccharides, which could constitute another biological carbon reservoir. It has been reported that few cells in trichomes of Arthrospira sp. PCC 8005 still display a high level of fluorescence after a long-term nitrogen starvation. The transmission electron microscopy showed that some cells still contained thylakoids and phycobilisomes after this long-term nitrogen starvation, which could explain the remaining fluorescence. |
| Databáze: | OpenAIRE |
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